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A Preliminary Study On Oocyte Collection Using Transvaginal Ultrasound In Swamp Buffalo Heifers (Bubalas Bubalis), Akachart Promdireg, Mongkol Techakumphu, Nawapen Phutikanit, Ancharlie Na-Chiangmai
A Preliminary Study On Oocyte Collection Using Transvaginal Ultrasound In Swamp Buffalo Heifers (Bubalas Bubalis), Akachart Promdireg, Mongkol Techakumphu, Nawapen Phutikanit, Ancharlie Na-Chiangmai
The Thai Journal of Veterinary Medicine
The objective of the study was to investigate the possibility of collecting oocytes using transvaginal ultrasound-guidance, in swamp buffalo heifers, after gonadotropin stimulation. Five swamp buffalo heifers were given a single dose of 3,000 IU of Pregnant Mare Serum Gonadotropin (PMSG) intramuscularly in order to stimulate the ovaries. Examination of the ovarian responses were carried out 48h after stimulation, the follicles were subsequently punctured and the oocytes examine under a stereomicroscope. The results of the ovarian response were 6.6 ± 1.1 follicles per animal with an average diameter of 7.1 ± 4.1 mm. The mean number of oocytes collected per …