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The Diagnosis Of Canine Distemper Disease Using Rt-Pcr, Weerawan Tiwananthakorn, Sirichai Junsuwanaruk, Anudep Rungsipipat, Achariya Sailasuta, Somporn Techangamsuwan, Sapasiri Siripornprasert, Susumu Tateyama, Ryoji Yamaguchi, Kazuyuki Uchida
The Diagnosis Of Canine Distemper Disease Using Rt-Pcr, Weerawan Tiwananthakorn, Sirichai Junsuwanaruk, Anudep Rungsipipat, Achariya Sailasuta, Somporn Techangamsuwan, Sapasiri Siripornprasert, Susumu Tateyama, Ryoji Yamaguchi, Kazuyuki Uchida
The Thai Journal of Veterinary Medicine
The diagnosis of canine distemper (CD) by a reverse transcriptase polymerase chain reaction (RT-PCR) technique using 2 sets of primer which were specific to two regions on the cDNA of nucleocapsid protein (NP) gene of the canine distemper virus (CDV), Onderstepoort strain. Together with pathology and immunohistochemistry, the RT-PCR test was used to confirm the diagnosis of canine distemper in 20 dogs. Histopathological examination revealed that 70% (14/20) had pneumonia, 50% (10/20) had non-suppurative encephalitis, 33% (7/20) had splenic follicular necrosis and 35% (7/ 20) had gastroenteritis. Immunohistochemical detection of CDV antigens in the brain, lungs, and the gastrointestinal mucosa …