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Porcine Intestinal Epithelial Cell Lines As A New In Vitro Model For Studying Adherence And Pathogenesis Of Enterotoxigenic Escherichia Coli, Seung Y. Koh, Sajan George, Volker Brozel, Rodney A. Moxley, David H. Francis, Radhey Kaushik

School of Veterinary and Biomedical Sciences: Faculty Publications

Enterotoxigenic Escherichia coli (ETEC) infections result in large economic losses in the swine industry worldwide. The organism causes diarrhea by adhering to and colonizing enterocytes in the small intestines. While much progress has been made in understanding the pathogenesis of ETEC, no homologous intestinal epithelial cultures suitable for studying porcine ETEC pathogenesis have been described prior to this report. In the current study, we investigated the adherence of various porcine ETEC strains to two porcine (IPEC-1 and IPEC-J2) and one human (INT-407) small intestinal epithelial cell lines. Each cell line was assessed for its ability to support the adherence of …