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Phenotypic Characterization Of Canine Cranial Cruciate Ligament Associated Synoviocytes, Sunil C. Vasanjee

LSU Master's Theses

Identification of synoviocytes surrounding the canine cranial cruciate ligament (CrCL) has not been investigated. Objectives 1) Develop and validate a technique to identify and quantify normal canine CrCL associated synoviocytes. 2) Compare synoviocyte phenotype proportions surrounding normal and abnormal canine CrCLs Design In vitro experimental Animals Cranial cruciate ligaments from 4 intact female and 6 intact adult male mixed-breed dogs (objective 1) and from 8 adult female hound dogs (objective 2) Methods Objective 1) Normal CrCLs - CD18 and HSP25 epitopes were colocalized using immunohistochemistry. Sagittal sections were quantified in the proximal, middle, and distal aspects of each section. Western …