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Medicine and Health Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Veterinary Medicine

TÜBİTAK

2023

PCR

Articles 1 - 2 of 2

Full-Text Articles in Medicine and Health Sciences

Prevalence Of Carriers Of Cholesterol Deficiency–Associated Apob Mutation In Some Native, Holstein And Simmental Cattle Breeds In Türkiye, Hasan Meydan, Kübra Bedi̇r Di̇bi̇ç, Onur Vural, Mehmet Ali̇ Yildiz, Jørgen S. Agerholm Dec 2023

Prevalence Of Carriers Of Cholesterol Deficiency–Associated Apob Mutation In Some Native, Holstein And Simmental Cattle Breeds In Türkiye, Hasan Meydan, Kübra Bedi̇r Di̇bi̇ç, Onur Vural, Mehmet Ali̇ Yildiz, Jørgen S. Agerholm

Turkish Journal of Veterinary & Animal Sciences

Genetic diseases play an increasingly important role in cattle breeding as breeding technologies such as artificial insemination has an inborn risk of disseminating deleterious genes, especially from high merit breeding sires. Cholesterol deficiency (CD) is a metabolic disorder first recognized in Holstein cattle in 2015. The condition is characterized by chronic diarrhea and unthriftiness in calves being homozygous for the defective allele. The prevalence of carriers of CD in Turkish cattle is at present unknown and therefore 700 heifers and cows of different breeds, including 300 Holsteins were genotyped. Of the tested Holsteins, 2.0% turned out to be carriers of …


Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak Jan 2023

Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to examine Canine Herpesvirus-1 (CHV-1) with different diagnostic methods and investigate the pathological changes in positive samples. For this purpose, 100 dog uteruses of different ages and breeds, were removed by ovariohysterectomy and their blood samples were used as the study material. Enzyme-linked immunosorbent assay (ELISA) and Indirect Fluorescent Antibody Test (IFAT) for serological diagnosis, polymerase chain reaction (PCR) method for molecular diagnosis, immunohistochemistry (IHC) and immunofluorescence (IF) staining for immunopathological diagnosis were performed on the samples. Additionally, hematoxylin and eosin (HE) staining was performed to examine the histopathological changes in the CHV- 1-positive …