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Full-Text Articles in Medicine and Health Sciences
Comparative Diagnostic Methods For Canine Ehrlichiosis, Chetan Parmar, Riddhi Pednekar, Anant Jayraw, Mukulesh Gatne
Comparative Diagnostic Methods For Canine Ehrlichiosis, Chetan Parmar, Riddhi Pednekar, Anant Jayraw, Mukulesh Gatne
Turkish Journal of Veterinary & Animal Sciences
The efficacy of blood and buffy coat smear examination, commercially available dot-enzyme-linked immunosorbent assay (ELISA) kit, and nested polymerase chain reaction (PCR) was evaluated in the diagnosis of canine ehrlichiosis in 40 dogs exhibiting symptoms suggestive of the disease. The intracytoplasmic rickettsial organisms were seen in the smears of only 1 dog (2.5%) with an extremely low level of parasitemia. Nested PCR with 16S rRNA revealed characteristic 378 bp bands indicating positive reaction in 8 (20.0%) dogs and sera samples of 21 (52.5%) dogs revealed anti-Ehrlichia antibodies. The dog that revealed organisms in the blood also showed positive results with …
Scrapie Cases In The Northern Cyprus, Aydin Gürel, Ahmet Gülçubuk, Nuri̇ Turan, Christopher Richard Helps, Hüseyi̇n Yilmaz
Scrapie Cases In The Northern Cyprus, Aydin Gürel, Ahmet Gülçubuk, Nuri̇ Turan, Christopher Richard Helps, Hüseyi̇n Yilmaz
Turkish Journal of Veterinary & Animal Sciences
Scrapie is a fatal neurodegenerative disease affecting small ruminants that causes economic losses in many countries. It is categorized together with other fatal neurodegenerative diseases as a transmissible spongiform encephalopathy. The aim of this study was to investigate clinical cases and the cause of death in a flock of sheep from Northern Cyprus (Turkish Republic of Northern Cyprus) suspected of having scrapie. Brains from 4 sheep showing clinical signs of classical scrapie, out of a flock of 200 animals, were analyzed by histopathological examination and infective prion enzyme-linked immunosorbent assay (ELISA). The 4 affected sheep were 2-3 years of age …
Immunokinetics Of Theileria Equi Specific Antibodies: A Comparison In Serial And Single Dilution Elisa Antibody End Titres, Yogesh Kumar, Dharam Vir Malhotra, Anil Kumar Nichani, Ashok Kumar, Shruti Dhar, Sanjay Kumar
Immunokinetics Of Theileria Equi Specific Antibodies: A Comparison In Serial And Single Dilution Elisa Antibody End Titres, Yogesh Kumar, Dharam Vir Malhotra, Anil Kumar Nichani, Ashok Kumar, Shruti Dhar, Sanjay Kumar
Turkish Journal of Veterinary & Animal Sciences
Theileria equi, a haemoprotozoan of equids, is responsible for endemic infection/latency in tropical/subtropical parts of the world. The present study investigated the immunokinetics of T. equi infection in experimental animals and the diagnostic applicability of single dilution ELISA vis-à-vis conventional serial dilution ELISA. Sequential serum samples were collected up to 90 days postinfection (PI) from 4 donkeys experimentally infected with T. equi. Immunokinetics of T. equi antibodies end titre were determined by serial and single dilution ELISA. A significant rise in initial ELISA antibody end titre (1:800 in serial dilution ELISA and from 1:600 to 1:650 in single dilution ELISA) …
Evaluation Of Canine Anti-Leishmania Igg Subclasses And Their Relation With Skin Signs In Naturally Infected Dogs In The Northwest Of Iran, Ali Shabestari Asl, Majid Sharifi, Mehdi Mohebali, Zabihollah Zarei, Zohreh Babaloo, Behnaz Akhoundi
Evaluation Of Canine Anti-Leishmania Igg Subclasses And Their Relation With Skin Signs In Naturally Infected Dogs In The Northwest Of Iran, Ali Shabestari Asl, Majid Sharifi, Mehdi Mohebali, Zabihollah Zarei, Zohreh Babaloo, Behnaz Akhoundi
Turkish Journal of Veterinary & Animal Sciences
Canine leishmaniasis (CanL) is a severe, intracellular parasitic zoonosis and is an endemic complaint in Iran. Dogs are considered the reservoir host for Leishmania infantum in the Old World. The aims of this study were to evaluate the relation between anti-Leishmania antibody titers (IgG) and skin symptoms of CanL in naturally affected dogs and to assess the correlation of IgG_1 and IgG_2 subclasses of titers with these symptoms. Anti-Leishmania antibody titers were evaluated in 100 dogs' blood samples with the direct agglutination test (DAT) and enzyme-linked immunosorbent assay (ELISA) methods. Based on DAT results, 45 samples were positive. Twenty-five of …