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Medicine and Health Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Veterinary Medicine

TÜBİTAK

Journal

2018

Polymerase chain reaction

Articles 1 - 2 of 2

Full-Text Articles in Medicine and Health Sciences

Survey Of Dirofilaria Immitis In Dogs From Sivas Province In Thecentral Anatolia Region Of Turkey, Ahmet Duran Ataş, Kürşat Altay, Ahmet Ali̇m, Erkan Özkan Jan 2018

Survey Of Dirofilaria Immitis In Dogs From Sivas Province In Thecentral Anatolia Region Of Turkey, Ahmet Duran Ataş, Kürşat Altay, Ahmet Ali̇m, Erkan Özkan

Turkish Journal of Veterinary & Animal Sciences

In this research, we investigate the prevalence of Dirofilaria immitis infection in dogs using microscopical, serological, and molecular methods from the Central Anatolia Region (Sivas) of Turkey and reveal the associated risk factors. Whole blood and serum samples were obtained from 306 dogs older than 7 months. Polymerase chain reaction (PCR) and the modified Knott's method (Knott) were used for detecting the microfilaria and enzyme-linked immunosorbent assay (ELISA) was applied for the presence of D. immitis antigens. The prevalence of D. immitis in dogs was detected as 2.9% (9/306) by PCR and ELISA and as 1.3% (4/306) by Knott. All …


A Case Report Of Apatemon Gracilis (Szidat, 1928) Infectionin Domestic Geese In Mainland China, Dandan Liu, Zhennan Zhuo, Jianping Tao, Jinjun Xu Jan 2018

A Case Report Of Apatemon Gracilis (Szidat, 1928) Infectionin Domestic Geese In Mainland China, Dandan Liu, Zhennan Zhuo, Jianping Tao, Jinjun Xu

Turkish Journal of Veterinary & Animal Sciences

This is the first report of the diagnosis and treatment of Apatemon gracilis infection in domestic geese in mainland China confirmed at the molecular level. The mortality of geese reached 10% (150/1500). Necropsy examinations confirmed hemorrhagic inflammation of the small intestine and blood and catarrhal mucus in the enteric cavity. A large number of flukes were found in the first third of the small intestine, with one end buried in the intestinal mucosa, forming lesions at the absorption sites. The ITS-1 sequence of the parasites was amplified by polymerase chain reaction and sequence analysis confirmed that the isolated worms were …