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Full-Text Articles in Medicine and Health Sciences
Analysis Of The Virulence Of Streptococcus Mutans Serotype C Gtfa Mutants In The Rat Model System, Raul G. Barletta, Suzanne M. Michalek, Roy Curtis Iii
Analysis Of The Virulence Of Streptococcus Mutans Serotype C Gtfa Mutants In The Rat Model System, Raul G. Barletta, Suzanne M. Michalek, Roy Curtis Iii
School of Veterinary and Biomedical Sciences: Faculty Publications
The Streptococcus mutans serotype c gtfA gene encodes a 55-kilodalton protein which catalyzes the synthesis of a small glucan (1.5 kilodaltons) from sucrose (J. P. Robeson, R. G. Barletta, and R. Curtiss III, J. Bacteriol. 153:211-221, 1983). To investigate the role of the GtfA enzyme in virulence, we constructed S. mutans gtfA mutants from three cariogenic serotype c strains. A plasmid that carried an erythromycin resistance determinant and an internal fragment of the gtfA gene but that was unable to replicate in streptococci was used to transform S. mutans. The erythromycin-resistant transformants carried a partial duplication of the internal …
Purification Of Maturation-Promoting Factor, An Intracellular Regulator Of Early Mitotic Events, Manfred J. Lohka, Marianne K. Hayes, James L. Maller
Purification Of Maturation-Promoting Factor, An Intracellular Regulator Of Early Mitotic Events, Manfred J. Lohka, Marianne K. Hayes, James L. Maller
School of Veterinary and Biomedical Sciences: Faculty Publications
Maturation-promoting factor causes germinal vesicle breakdown when injected into Xenopus oocytes and can induce metaphase in a cell-free system. The cell-free assay was used to monitor maturation-promoting factor during its purification from unfertilized Xenopus eggs. Ammonium sulfate precipitation and six chromatographic procedures resulted in a preparation purified >3000-fold that could induce germinal vesicle breakdown within 2 hr when injected into cycloheximide- treated oocytes. Proteins of 45 kDa and 32 kDa were correlated with fractions of highest activity in both assays. These fractions contained a protein kinase activity able to phosphorylate the endogenous 45-kDa protein, as well as histone Hi, phosphatase …
Improved Selective Medium For The Isolation Of Treponema Hyodysenteriae, Robert A. Kunkle, J. M. Kinyon
Improved Selective Medium For The Isolation Of Treponema Hyodysenteriae, Robert A. Kunkle, J. M. Kinyon
School of Veterinary and Biomedical Sciences: Faculty Publications
An agar medium with improved selection for Treponema hyodysenteriae was developed. Cultures of T. hyodysenteriae and T. innocens, feces from 11 clinically normal pigs, and colonic contents from 6 pigs with gross lesions consistent with swine dysentery were diluted in phosphate-buffered saline and plated on Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.) with 5% citrated bovine blood (TSA), TSA with 400 ,ug of spectinomycin per ml (TSA-S400), TSA-S400 with 25 ,ug each of colistin and vancomycin per ml, and TSA with 5% pig feces extract and five antimicrobial agents (spiramycin, rifampin, vancomycin, colistin, and spectinomycin) (BJ). Viable numbers …