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Full-Text Articles in Medicine and Health Sciences
Ultraviolet Action Spectroscopy Of Iodine Labeled Peptides And Proteins In The Gas Phase, Benjamin B. Kirk, Adam J. Trevitt, Stephen J. Blanksby, Yuanqi Tao, Benjamin N. Moore, Ryan Julian
Ultraviolet Action Spectroscopy Of Iodine Labeled Peptides And Proteins In The Gas Phase, Benjamin B. Kirk, Adam J. Trevitt, Stephen J. Blanksby, Yuanqi Tao, Benjamin N. Moore, Ryan Julian
Adam Trevitt
Structural investigations of large biomolecules in the gas phase are challenging. Herein, it is reported that action spectroscopy taking advantage of facile carbon-iodine bond dissociation can be used to examine the structures of large molecules, including whole proteins. Iodotyrosine serves as the active chromophore, which yields distinctive spectra depending on the solvation of the side chain by the remainder of the molecule. Isolation of the chromophore yields a double featured peak at 290 nm, which becomes a single peak with increasing solvation. Deprotonation of the side chain also leads to reduced apparent intensity and broadening of the action spectrum. The …
Uv Photodissociation Action Spectroscopy Of Haloanilinium Ions In A Linear Quadrupole Ion Trap Mass Spectrometer, Christopher S. Hansen, Benjamin B. Kirk, Stephen J. Blanksby, Richard A. J O'Hair, Adam J. Trevitt
Uv Photodissociation Action Spectroscopy Of Haloanilinium Ions In A Linear Quadrupole Ion Trap Mass Spectrometer, Christopher S. Hansen, Benjamin B. Kirk, Stephen J. Blanksby, Richard A. J O'Hair, Adam J. Trevitt
Adam Trevitt
UV-vis photodissociation action spectroscopy is becoming increasingly prevalent because of advances in, and commercial availability of, ion trapping technologies and tunable laser sources. This study outlines in detail an instrumental arrangement, combining a commercial ion-trap mass spectrometer and tunable nanosecond pulsed laser source, for performing fully automated photodissociation action spectroscopy on gas-phase ions. The components of the instrumentation are outlined, including the optical and electronic interfacing, in addition to the control software for automating the experiment and performing online analysis of the spectra. To demonstrate the utility of this ensemble, the photodissociation action spectra of 4-chloroanilinium, 4-bromoanilinium, and 4-iodoanilinium cations …