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Hormonal Regulation Of Lipolysis And Phosphorylase Activity In Human Fat Cells, Jay Moskowitz, John N. Fain

Faculty Publications

A B S TR A C T Prostaglandin E₁ (PGE₁) at a concentration of 1 ng/ml antagonized theophylline, and norepinephrine induced release of glycerol and free fatty acids (FFA) in human fat cell preparations. Insulin at higher doses also inhibited theophylline-stimulated lipolysis. The N₆-2-O^'dibutyryl derivative of cyclic adenosine monophosphate (DCAMP) stimulated lipolysis. Prostaglandin E1 did not significantly inhibit the lipid mobilizing effects of DCAMP. Changes in glycogen phosphorylase activity after treatment with theophylline, norepinephrine, DCAMP, and PGE₁ paralleled those of lipolysis. These results suggest that in man as in experimental animals lipolysis …