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Cloning And Sequencing Of The Genes For Shiga Toxin From Shigella Dysenteriae Type 1, Nancy A. Strockbine, Matthew Jackson, Lawrence Sung, Randall Holmes, Alison D. O'Brien
Cloning And Sequencing Of The Genes For Shiga Toxin From Shigella Dysenteriae Type 1, Nancy A. Strockbine, Matthew Jackson, Lawrence Sung, Randall Holmes, Alison D. O'Brien
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The structural genes for Shiga toxin, designated stx A and stx B, were cloned from Shigella dysenteriae type 1 3818T, and a nucleotide sequence analysis was performed. Both stx A and stx B were present on a single transcriptional unit, with stx A preceding stx B. The molecular weight calculated for the processed A subunit was 32,225, while the molecular weight of the processed B subunit was 7,691. Comparison of the nucleotide sequences for Shiga toxin and Shiga-like toxin I (SLT-I) from Escherichia coli revealed that the genes for Shiga toxin and SLT-I were greater than 99% homologous; three nucleotide …