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The Transcriptional Response To Oxidative Stress During Vertebrate Development: Effects Of Tert-Butylhydroquinone And 2,3,7,8-Tetrachlorodibenzo-P-Dioxin, Mark E. Hahn, Andrew G. Mcarthur, Sibel I. Karchner, Diana G. Franks, Matthew J. Jenny, Alicia R. Timme-Laragy, John J. Stegeman, Bruce R. Woodin, Michael J. Cipriano, Elwood Linney

Alicia R. Timme-Laragy

Oxidative stress is an important mechanism of chemical toxicity, contributing to teratogenesis and to cardiovascular and neurodegenerative diseases. Developing animals may be especially sensitive to chemicals causing oxidative stress. The developmental expression and inducibility of anti-oxidant defenses through activation of NF-E2-related factor 2 (NRF2) affect susceptibility to oxidants, but the embryonic response to oxidants is not well understood. To assess the response to chemically mediated oxidative stress and how it may vary during development, zebrafish embryos, eleutheroembryos, or larvae at 1, 2, 3, 4, 5, and 6 days post fertilization (dpf) were exposed to DMSO (0.1%), tert-butylhydroquinone (tBHQ; 10 µM) …