Medicine and Health Sciences Commons^™

Kaempferol-Phospholipid Complex: Formulation, And Evaluation Of Improved Solubility, In Vivo Bioavailability, And Antioxidant Potential Of Kaempferol, Darshan R. Telange, Arun T. Patil, Anil M. Pethe, Amol A. Tatode, Sridhar Anand, Vivek S. Dave

Pharmacy Faculty/Staff Publications

The current work describes the formulation and evaluation of a phospholipid complex of kaempferol toenhance the latter’s aqueous solubility, in vitro dissolution rate, in vivo antioxidant and hepatoprotectiveactivities, and oral bioavailability. The kaempferol-phospholipid complex was synthesized using a freeze-drying method with the formulation being optimized using a full factorial design (32) approach. The resultsinclude the validation of the mathematical model in order to ascertain the role of specific formulation andprocess variables that contribute favorably to the formulation’s development. The final product wascharacterized and confirmed by Differential Scanning Calorimetry (DSC), Fourier Transform InfraredSpectroscopy (FTIR), Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), and …

Formulation And Characterization Of An Apigenin-Phospholipid Phytosome (Aplc) For Improved Solubility, In Vivo Bioavailability, And Antioxidant Potential, Darshan R. Telange, Arun T. Patin, Anil M. Pethe, Harshal Fegade, Sridhar Anand, Vivek S. Dave

Pharmacy Faculty/Staff Publications

The apigenin-phospholipid phytosome (APLC) was developed to improve the aqueous solubility, dissolution, in vivo bioavailability, and antioxidant activity of apigenin. The APLC synthesis was guided by a full factorial design strategy, incorporating specific formulation and process variables to deliver an optimized product. The design-optimized formulation was assayed for aqueous solubility, in vitro dissolution, pharmacokinetics, and antioxidant activity. The pharmacological evaluation was carried out by assessing its effects on carbon tetrachloride-induced elevation of liver function marker enzymes in a rat model. The antioxidant activity was assessed by studying its effects on the liver antioxidant marker enzymes. The developed model was validated …

Medication Adherence In Patients With Myotonic Dystrophy And Facioscapulohumeral Muscular Dystrophy, Bryan Fitzgerald, Kelly Conn, Joanne Smith, Andrew Walker, Amy L. Parkhill, James E. Hilbert, Elizabeth A. Luebbe, Richard T. Moxley Iii

Doctoral External Publications

Myotonic dystrophy (DM) and facioscapulohumeral muscular dystrophy (FSHD) are the two most common adult muscular dystrophies and have progressive and often disabling manifestations. Higher levels of medication adherence lead to better health outcomes, especially important to patients with DM and FSHD because of their multisystem manifestations and complexity of care. However, medication adherence has not previously been studied in a large cohort of DM type 1 (DM1), DM type 2 (DM2), and FSHD patients. The purpose of our study was to survey medication adherence and disease manifestations in patients enrolled in the NIH-supported National DM and FSHD Registry. The study …

Fructose Alters Cell Survival And Gene Expression In Microglia And Neuronal Cells Lines, Drew P. Seidel, Susan Denapoli, Thomas S. Maciulewicz, Melinda E. Lull

Pharmacy Faculty/Staff Publications

Purpose: Microglia are macrophages that are found primarily in the CNS and play a crucial role in maintaining a healthy brain by engulfing invading microorganisms, releasing inflammatory mediators, and pruning dead cells. Microglia can become activated in response to certain stimuli which causes them to transition into a pro-inflammatory state, and can sometimes become chronically activated which can result in neuronal damage. Studies have shown a causal relationship between this activation and sugars such as fructose and glucose. We sought to understand the role of sugars in microglial activation and the subsequent effects on neuron health.

Methods: Rat microglia (HAPI) …

Online Self-Testing Resources Prepared By Peer Tutors As A Formative Assessment Tool In Pharmacology Courses, Melinda E. Lull, Jennifer Mathews

Pharmacy Faculty/Staff Publications

Objective. To assess the effectiveness of optional online quizzes written by peer tutors in a pharmacology course for doctor of pharmacy students.

Methods. Online quizzes were written by peer tutors for second-year pharmacy students. Quizzes reflected the material taught during lecture and were in a format similar to that of the examinations. Data related to performance on each quiz and each examination were collected throughout the semester. At the end of the semester, students and peer tutors were surveyed to gather information on the utility and success of the quizzes.

Results. Students taking online quizzes performed significantly better on examinations …