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Antigen expression; antigen purification; article; binding affinity; carboxy terminal sequence; cellular distribution; confocal microscopy; controlled study; drug efficacy; endoplasmic reticulum; female; glycosylation; Golgi complex; human; human cell; hybridoma; immunoblotting; in vitro study; in vivo study; mouse; nonhuman; nuclear reprogramming; plant cell; plant leaf; protein domain; protein localization; protein purification; quantitative analysis; Rabies virus; real time polymerase chain reaction; signal transduction; tobacco; transcription regulation; trend study; virus neutralization
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Full-Text Articles in Medicine and Health Sciences
Intracellular Reprogramming Of Expression, Glycosylation, And Function Of A Plant-Derived Antiviral Therapeutic Monoclonal Antibody., Jeong-Hwan Lee, Da-Young Park, Kyung-Jin Lee, Young-Kwan Kim, Yang-Kang So, Jae-Sung Ryu, Seung-Han Oh, Yeon-Soo Han, Kinarm Ko, Young-Kug Choo, Sung-Joo Park, Robert Brodzik, Kyoung-Ki Lee, Doo-Byoung Oh, Kyung-A Hwang, Hilary Koprowski, Yong Seong Lee, Kisung Ko
Intracellular Reprogramming Of Expression, Glycosylation, And Function Of A Plant-Derived Antiviral Therapeutic Monoclonal Antibody., Jeong-Hwan Lee, Da-Young Park, Kyung-Jin Lee, Young-Kwan Kim, Yang-Kang So, Jae-Sung Ryu, Seung-Han Oh, Yeon-Soo Han, Kinarm Ko, Young-Kug Choo, Sung-Joo Park, Robert Brodzik, Kyoung-Ki Lee, Doo-Byoung Oh, Kyung-A Hwang, Hilary Koprowski, Yong Seong Lee, Kisung Ko
Department of Cancer Biology Faculty Papers
Plant genetic engineering, which has led to the production of plant-derived monoclonal antibodies (mAb(P)s), provides a safe and economically effective alternative to conventional antibody expression methods. In this study, the expression levels and biological properties of the anti-rabies virus mAb(P) SO57 with or without an endoplasmic reticulum (ER)-retention peptide signal (Lys-Asp-Glu-Leu; KDEL) in transgenic tobacco plants (Nicotiana tabacum) were analyzed. The expression levels of mAb(P) SO57 with KDEL (mAb(P)K) were significantly higher than those of mAb(P) SO57 without KDEL (mAb(P)) regardless of the transcription level. The Fc domains of both purified mAb(P) and mAb(P)K and hybridoma-derived mAb (mAb(H)) had similar …