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Rapid Selection And Proliferation Of Cd133(+) Cells From Cancer Cell Lines: Chemotherapeutic Implications, Sarah Kelly, Altomare Benedetto, Adelaide Greco, Candace Howard, Vincent Sollars, Donald Primerano, Jagan Valluri, Pier Claudio
Rapid Selection And Proliferation Of Cd133(+) Cells From Cancer Cell Lines: Chemotherapeutic Implications, Sarah Kelly, Altomare Benedetto, Adelaide Greco, Candace Howard, Vincent Sollars, Donald Primerano, Jagan Valluri, Pier Claudio
Donald A Primerano
Cancer stem cells (CSCs) are considered a subset of the bulk tumor responsible for initiating and maintaining the disease. Several surface cellular markers have been recently used to identify CSCs. Among those is CD133, which is expressed by hematopoietic progenitor cells as well as embryonic stem cells and various cancers. We have recently isolated and cultured CD133 positive [CD133(+)] cells from various cancer cell lines using a NASA developed Hydrodynamic Focusing Bioreactor (HFB) (Celdyne, Houston, TX). For comparison, another bioreactor, the rotary cell culture system (RCCS) manufactured by Synthecon (Houston, TX) was used. Both the HFB and the RCCS bioreactors …