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The Nitric Oxide-Cyclic Gmp Pathway Plays An Essential Role In Both Promoting Cell Survival Of Cerebellar Granule Cells In Culture And Protecting The Cells Against Ethanol Neurotoxicity, N. Pantazis, J. West, Donghai Dai
The Nitric Oxide-Cyclic Gmp Pathway Plays An Essential Role In Both Promoting Cell Survival Of Cerebellar Granule Cells In Culture And Protecting The Cells Against Ethanol Neurotoxicity, N. Pantazis, J. West, Donghai Dai
Donghai Dai
NMDA has two beneficial effects on primary neuronal cultures of cerebellar granule cells (CGCs) established from 10-day-old rat pups. First, NMDA is neurotrophic and will enhance survival of CGCs in culture in the absence of ethanol. Second, ethanol exposure will induce cell death in CGC cultures, and NMDA can lessen this ethanol-induced cell loss, i.e., NMDA is neuroprotective. Because NMDA can stimulate production of nitric oxide (NO), which can in turn enhance synthesis of cyclic GMP, this study tested the hypothesis that the NO-cyclic GMP pathway is essential for NMDA-mediated neurotrophism and neuroprotection. Inhibiting the synthesis of NO with N(G)-nitro-L-arginine …
Fgf-2, Ngf And Igf-1, But Not Bdnf, Utilize A Nitric Oxide Pathway To Signal Neurotrophic And Neuroprotective Effects Against Alcohol Toxicity In Cerebellar Granule Cell Cultures, D. Bonthius, B. Karacay, Donghai Dai, N. Pantazis
Fgf-2, Ngf And Igf-1, But Not Bdnf, Utilize A Nitric Oxide Pathway To Signal Neurotrophic And Neuroprotective Effects Against Alcohol Toxicity In Cerebellar Granule Cell Cultures, D. Bonthius, B. Karacay, Donghai Dai, N. Pantazis
Donghai Dai
Neuronal death is a prominent neuropathological component of fetal alcohol syndrome (FAS). Identification of molecular agents and pathways that can ameliorate alcohol-induced cell loss offers possible therapeutic strategies for FAS and potential insight into its pathogenesis. This study investigated the effects of growth factors on cellular survival in alcohol-exposed cerebellar granule cell (CGC) cultures and examined the role of the nitric oxide (NO)-cGMP-PKG (cGMP-dependent protein kinase) pathway in the cell survival-promoting effects of these growth factors. Primary CGC cultures were exposed to 0 or 400 mg/dl ethanol, accompanied by either no growth factor or 30 ng/ml fibroblast growth factor-2 (FGF-2), …