Medicine and Health Sciences Commons^™

16s Rrna Gene-Based Analysis Of Fecal Microbiota From Preterm Infants With And Without Necrotizing Enterocolitis, Yunwei Wang, Jeanette D. Hoenig, Kathryn J. Malin, Sanaa Qamar, Elaine O. Petrof, Jun Sun, Dionysios A. Antonopoulos, Eugene B. Chang, Erika C. Claud

College of Nursing Faculty Research and Publications

Neonatal necrotizing enterocolitis (NEC) is an inflammatory intestinal disorder affecting preterm infants. Intestinal bacteria play a key role; however no causative pathogen has been identified. The purpose of this study was to determine if there are differences in microbial patterns which may be critical to the development of this disease. Fecal samples from twenty preterm infants, ten with NEC and ten matched controls (including four twin pairs) were obtained from patients in a single site Level III neonatal intensive care unit. Bacterial DNA from individual fecal samples were PCR amplified and subjected to terminal restriction fragment length polymorphism analysis and …