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Full-Text Articles in Medicine and Health Sciences
Evaluating Soluble Axl As A Biomarker For Glioblastoma, Daniel Raymond
Evaluating Soluble Axl As A Biomarker For Glioblastoma, Daniel Raymond
All NMU Master's Theses
AXL, a receptor tyrosine kinase, is known to promote malignant phenotypes in various types of cancer. AXL overexpression is commonly observed in glioblastoma and correlates with a worse prognosis. Detectable in most biological fluids, the extracellular domain of AXL, sAXL, is the product of enzymatic cleavage by ADAM10/17. Here we used ELISA, BCA and BCG assays to characterize blood serum from 23 newly diagnosed GBM patients which was collected roughly 24 hours before and after surgery as well as every three months post-surgery, corresponding to follow-up treatment visits. Immunoblotting was used to determine relative AXL expression in 13 patient tumor …
Characterization Of Hcmv-Encoded Chemokine Receptor Us28 Transduced Msu1.1 Human Fibroblast Cells, Danielle R. Vanbeckum
Characterization Of Hcmv-Encoded Chemokine Receptor Us28 Transduced Msu1.1 Human Fibroblast Cells, Danielle R. Vanbeckum
All NMU Master's Theses
Human Cytomegalovirus (HCMV) encodes the G-protein coupled receptor US28. Using a mouse model system, US28 was previously found to be oncomodulatory, increasing proliferation, inducing anchorage independent growth and loss of contact inhibition. Similarly, in the human glioblastoma cell line U373, US28 activated VEGF expression. To determine if US28 is oncomodulatory in normal human cells, we engineered the human fibroblast cell line MSU1.1 to express US28 via lentivirus infection. MSU1.1 cells were transduced with pHAGE-US28-ZsGreen. Fluorescent confocal microscopy was utilized to detect the ZsGreen tag and confirmed the successful transduction of MSU1.1 cells with the US28 vector or empty pHAGE vector. …