Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- 2D Gels (1)
- Agonists (1)
- Colorectal Neoplasms (1)
- Experimental Design (1)
- False Discovery Rate (1)
-
- Gene Expression Profiling (1)
- HCT116 Cells (1)
- Human (1)
- Laser Capture (1)
- Library research in the Geosciences (1)
- Mass Spectrometry (1)
- Neoplastic drug effects (1)
- PPAR gamma physiology (1)
- Pharmacology (1)
- Prostaglandin D2 (1)
- Proteomics (1)
- Randomization (1)
- Regulation (1)
- Reverse Transcriptase Polymerase Chain Reaction (1)
- Signal Transduction (1)
- Thiazolidinediones (1)
- Transcriptional Activation (1)
- File Type
Articles 1 - 3 of 3
Full-Text Articles in Medicine and Health Sciences
Gene Alterations By Peroxisome Proliferator-Activated Receptor Gamma Agonists In Human Colorectal Cancer Cells, Maria Cekanova, J Yuan, X Li, K B. Kim, Seung J. Baek
Gene Alterations By Peroxisome Proliferator-Activated Receptor Gamma Agonists In Human Colorectal Cancer Cells, Maria Cekanova, J Yuan, X Li, K B. Kim, Seung J. Baek
Maria Cekanova MS, RNDr, PhD
The peroxisome proliferator-activated receptor gamma (PPARgamma) is a nuclear transcription factor that controls the genes involved in metabolism and carcinogenesis. In the present study, we examined the alteration of gene expression in HCT-116 human colorectal cancer cells by PPARgamma agonists: MCC-555 (5 microM), rosiglitazone (5 microM), and 15-deoxy-Delta12,14-prostaglandin J2 (1 microM). The long-oligo microarray data revealed a list of target genes commonly induced (307 genes) and repressed (32 genes) by tested PPARgamma agonists. These genes were analyzed by Onto-Express software and KEGG pathway analysis and revealed that PPARgamma agonists are involved in cell proliferation, focal adhesion, and several signaling pathways. …
Microproteomics: Analysis Of Protein Diversity In Small Samples, Howard B. Gutstein, Jeffrey S. Morris, Suresh P. Annangudi, Jonathan V. Sweedler
Microproteomics: Analysis Of Protein Diversity In Small Samples, Howard B. Gutstein, Jeffrey S. Morris, Suresh P. Annangudi, Jonathan V. Sweedler
Jeffrey S. Morris
Proteomics, the large-scale study of protein expression in organisms, offers the potential to evaluate global changes in protein expression and their post-translational modifications that take place in response to normal or pathological stimuli. One challenge has been the requirement for substantial amounts of tissue in order to perform comprehensive proteomic characterization. In heterogeneous tissues, such as brain, this has limited the application of proteomic methodologies. Efforts to adapt standard methods of tissue sampling, protein extraction, arraying, and identification are reviewed, with an emphasis on those appropriate to smaller samples ranging in size from several microliters down to single cells. The …
Important, But Odd And Obscure, Reasons To Use The Library, Maxine G. Schmidt
Important, But Odd And Obscure, Reasons To Use The Library, Maxine G. Schmidt
Maxine G Schmidt
No abstract provided.