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Capturing Variation In Lens (Fabaceae): Development And Utility Of An Exome Capture Array For Lentil, Ezgi Ogutcen, Larissa Ramsay, Eric Bishop Von Wettberg, Kirstin E. Bett

College of Agriculture and Life Sciences Faculty Publications

Premise of the Study: Lentil is an important legume crop with reduced genetic diversity caused by domestication bottlenecks. Due to its large and complex genome, tools for reduced representation sequencing are needed. We developed an exome capture array for use in various genetic diversity studies. Methods: Based on the CDC Redberry draft genome, we developed an exome capture array using multiple sources of transcript resources. The probes were designed to target not only the cultivated lentil, but also wild species. We assessed the utility of the developed method by applying the generated data set to population structure and phylogenetic analyses. …