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A Thermodynamic Study Of Electroneutral K-Cl Cotransport In Ph- And Volume-Clamped Low K Sheep Erythrocytes With Normal And Low Internal Magnesium, Peter K. Lauf, Norma C. Adragna

Pharmacology and Toxicology Faculty Publications

Swelling-Induced human erythrocyte K-Cl cotransport is membrane potential independent and capable of uphill transport. However, a complete thermodynamic analysis of basal and stimulated K-Cl cotransport, at constant cell volume, is missing, This study was performed in low K sheep red blood cells before and after reducing cellular free Mg into the nanomolar range with the divalent cation ionophore A23187 and a chelator, an intervention known to stimulate K-Cl cotransport. The anion exchange inhibitor 4,4'diisothiocyanato-2,2'disulfonic stilbene was used to clamp intracellular pH and Cl or NO3 concentrations. Cell volume was maintained constant as external and internal pH differed by more than …