Medicine and Health Sciences Commons^™

Myeloid Dendritic Cells Of Patients With Chronic Hcv Infection Induce Proliferation Of Regulatory T Lymphocytes, Angela Dolganiuc, Edward Paek, Karen Kodys, Joanne Thomas, Gyongyi Szabo

Gyongyi Szabo

BACKGROUND and AIMS: Dendritic cells (DCs) initiate and sustain an efficient T-lymphocyte response. Chronic hepatitis C virus (HCV) infection is associated with inefficient T-cell functions that fail to eradicate the virus, so defects in DC function might be involved in HCV pathogenesis. This study analyzed the activities of myeloid DCs and distinct CD4(+) T-cell populations in samples collected from patients with HCV. METHODS: The abilities of primary BDCA1(+) or monocyte-derived DCs from HCV patients (HCV-DC) to stimulate CD4(+), CD4(+)CD25(-), or different ratios of CD4(+)CD25(+)/CD4(+)CD25(-) T cells were evaluated in mixed lymphocyte reactions. T-cell proliferation and phenotype were evaluated by flow …