Medicine and Health Sciences Commons^™

Beta-Adrenergic, Arachidonic Acid And Potassium Channel Associated-Regulation Of Human Breast Cancer Cell Lines, Yavuz Cakir

Doctoral Dissertations

During the late stages of breast cancer progression, breast cancer cell growth switches from steroid hormone to growth factor dependence, and it is assumed that invasive breast cancers are growth factor receptor positive and estrogen hormone resistant. The cellular arachidonic acid (AA) pathway is upregulated in a variety of cancer types, and it may play an important role in the growth regulation of breast cancer cells.

Recent evidence suggests that beta-adrenergic receptors (β-ARs) are expressed in some estrogen receptor (ER) negative breast cancers and that beta agonists not only can trigger AA release via the activation of cytosolic PLA2 or …

Abnormal Expression Of P27kip1 Protein In Levator Ani Muscle Of Aging Women With Pelvic Floor Disorders – A Relationship To The Cellular Differentiation And Degeneration, Antonin Bukovsky, Pleas Copas, Michael R. Caudle, Maria Cekanova, Tamara Dassanayake, Bridgett Asbury, Stuart E. Van Meter, Robert F. Elder, Jeffrey B. Brown, Stephanie B. Cross

Faculty Publications and Other Works -- General Biology

Background

Pelvic floor disorders affect almost 50% of aging women. An important role in the pelvic floor support belongs to the levator ani muscle. The p27/kip1 (p27) protein, multifunctional cyclin-dependent kinase inhibitor, shows changing expression in differentiating skeletal muscle cells during development, and relatively high levels of p27 RNA were detected in the normal human skeletal muscles.

Methods

Biopsy samples of levator ani muscle were obtained from 22 symptomatic patients with stress urinary incontinence, pelvic organ prolapse, and overlaps (age range 38–74), and nine asymptomatic women (age 31–49). Cryostat sections were investigated for p27 protein expression and type I (slow …