Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Hair Cells (2)
- Microtubules (2)
- Protein Isoforms (2)
- Aging (1)
- Antibodies (1)
-
- Antibody Specificity (1)
- Auditory (1)
- Cell Compartmentation (1)
- Cell Differentiation (1)
- Cilia (1)
- Confocal (1)
- Dendrites (1)
- Electron (1)
- Female (1)
- Fluorescent Antibody Technique (1)
- Ganglia (1)
- Indirect (1)
- Inner (1)
- Isomerism (1)
- Labyrinth Supporting Cells (1)
- Male (1)
- Microscopy (1)
- Newborn (1)
- Organ Specificity (1)
- Organ of Corti (1)
- Outer (1)
- Sensory (1)
Articles 1 - 3 of 3
Full-Text Articles in Medicine and Health Sciences
Requirement For The Betai And Betaiv Tubulin Isotypes In Mammalian Cilia., Heather C Jensen-Smith, Richard F Ludueña, Richard Hallworth
Requirement For The Betai And Betaiv Tubulin Isotypes In Mammalian Cilia., Heather C Jensen-Smith, Richard F Ludueña, Richard Hallworth
Journal Articles: Eppley Institute
Nielsen et al., [2001: Curr Biol 11:529-533], based on studies in Drosophila, have proposed that beta tubulin in axonemal microtubules must contain a specific acidic seven amino acid sequence in its carboxyl terminus. In mammals, the two betaIV isotypes (betaIVa and betaIVb) contain that sequence. In order to test the application of this hypothesis to mammals, we have examined the expression of beta tubulin isotypes in four different ciliated tissues (trachea, ependyma, uterine tube, and testis) using isotype-specific antibodies and indirect immunofluorescence. We find that betaIV tubulin is present in all ciliated cell types examined, but so is betaI tubulin. …
Cell Type-Specific Reduction Of Beta Tubulin Isotypes Synthesized In The Developing Gerbil Organ Of Corti, Heather Jensen Smith, Jonquille Eley, Peter S. Steyger, Richard F. Ludueña, Richard Hallworth
Cell Type-Specific Reduction Of Beta Tubulin Isotypes Synthesized In The Developing Gerbil Organ Of Corti, Heather Jensen Smith, Jonquille Eley, Peter S. Steyger, Richard F. Ludueña, Richard Hallworth
Journal Articles: Eppley Institute
There are seven isotypic forms of the microtubule protein beta tubulin in mammals, but not all isotypes are synthesized in every cell type. In the adult organ of Corti, each of the five major cell types synthesizes a different subset of isotypes. Inner hair cells synthesize only betaI and betaII tubulin, while outer hair cells make betaI and betaIV tubulin. Only betaII and betaIV tubulin are found in inner and outer pillar cells, while betaI, betaII, and betaIV tubulin are present in Deiters cells, and betaI, betaII and betaIII tubulin are found in organ of Corti dendrites. During post-natal organ …
Selective Expression Of Beta Tubulin Isotypes In Gerbil Vestibular Sensory Epithelia And Neurons, Brian Perry, Heather Jensen Smith, Richard F. Ludueña, Richard Hallworth
Selective Expression Of Beta Tubulin Isotypes In Gerbil Vestibular Sensory Epithelia And Neurons, Brian Perry, Heather Jensen Smith, Richard F. Ludueña, Richard Hallworth
Journal Articles: Eppley Institute
The seven mammalian isotypes of beta tubulin are strikingly similar in amino acid sequence. The differences in isotypic sequence, although small, are nonetheless conserved in evolution, which suggests that they may confer distinct functional roles. If so, such roles should be reflected in the selective expression of isotypes by cell type, or even in the sorting of isotypes to within-cell pools. Hair cells of the vestibular sensory epithelia each possess a kinocilium, a microtubule-based organelle that could represent a distinct microtubule compartment, separate from the extensive microtubule network in the soma. The afferent neurons that innervate the vestibular sensory epithelia …