Medicine and Health Sciences Commons^™

Nitrous Oxide Adaptation On Gene Expression In Breast Cancer Cells, Harsh Chheda, Estevan Ruiz Limón López, Zainab Mahmood, Ankit Sharma, Tina Tran, Uzma Abdulbaseer, Farouk Alshatti, James A. Radosevich

Chemistry and Physics Faculty Proceedings, Presentations, Speeches, Lectures

Hypothesis and Objective: Genes participating in the notch signaling pathway are influenced by exposure to high nitrous oxide (HNO) levels. Notch signaling pathway genes are responsible for regulating cell fate, differentiation, and apoptosis. Dysregulation of these genes can contribute to tumorigenesis. Alterations in the expression of these genes in MCF7 breast cancer cells could help determine the effects of the notch signaling pathway on tumorigenesis as a result of exposure to nitrous oxide.

Methods: Samples of cDNA from MCF7 and MCF7-HNO cells were used in DNA microarray analysis. Qualifying genes of interest provided a p value < .05. Differentially expressed genes (DEGs) were defined and categorized using the Gene Oncology Uniprot (GO) Molecular Function database. These cells were further analyzed using a proprietary bioinformatics analysis created by our laboratory.

Results: Exposure to HNO …

Reduced Incidence Of New Gastrointestinal Cancers Diagnosis During Covid-19 Pandemic, Abhilash Perisetti Md, Syed Mahanazuddin Phd, Rachana Yendala Md, Hermant Goyal Md, Benjamin Tharian Md, Mariajose Rojas Md, Neil Sharma Md

PCI Publications and Projects

Introduction- Gastrointestinal cancers such as esophageal, gastric, colon, liver, and pancreas cancers present a significant global challenge. The burden of these cancers depends mainly on early recognition and treatment initiation. COVID-19 pandemic led to a strain on multiple healthcare systems resulting in stay-at-home restraints, which might have impacted early recognition of these cancers. The data about the the incidence of GI cancers during the pandemic is scarce.

Methods- All adult patients with a new diagnosis of GI cancers in 2020 were compared with the patients in 2019. ICD-10 codes were utilized to identify these patient from TriNetX, a federated cloud-based …

Effect Of Pandemic On Endoscopic Utilization In Colonic Volvulus: A Global Multicentric Study, Abhilash Perisetti Md, Hermant Goyal Md, Syed Mahanazuddin Phd, Saurabh Chandan Md, Ruthvik Allala Ba, Mariajose Rojas Md, Jonathan Kopel Phd, Sonali Sachdeva Mbbs, Mark M. Aloysius Md, Phd, Benjamin Tharian, Neil Sharma Md

Other Specialties

Introduction- Acute colonic volvulus (ACV) is a gastrointestinal emergency that results from the torsion of a segment of the colon either at the level of the sigmoid colon or cecum. It can lead to severe complications such as bowel infarction and peritonitis which can increase mortality. The COVID-19 pandemic has led to a significant reduction in endoscopic utilization globally. However, the data about endoscopy utilization in a gastrointestinal emergency such as ACV is not non-existent. Hence, we attempted to evaluate the rates of endoscopy utilization in 2020 compared to 2019 for ACV patients.

Methods- We used TriNetX, a federated cloud-based …

Safety Of Glucagon Use During Endoscopic Retrograde Cholangiopancreatography: A Retrospective Multicentric Study, Abhilash Perisetti Md, Hermant Goyal Md, Syed Mahanazuddin Phd, Rupinder Mann Md, Saurabh Chandan Md, Benjamin Tharian Md, Neil Sharma Md

Other Specialties

Introduction- Injectable glucagon inhibits gastrointestinal (GI) and duodenal motility by relaxing smooth muscles, decreasing the frequency and amplitude of phasic activity of the sphincter of Oddi. This enables endoscopists to enhance biliary cannulation and extract small bile duct stone. Additionally, glucagon can potentially worsen underlying hyperglycemia and hyperkalemia especially in patients with diabetes. It is unclear if glucagon impacts the adverse events of Endoscopic Retrograde Cholangiopancreatography (ERCP) such as rates of GI bleeding, GI perforation, post-ERCP pancreatitis (PEP) and in patient hospitalizations.

Methods We used TriNetX, a federated cloud-based network comprising of 92 healthcare organizations (HCOs) across the US. All …

Impact Of Pandemic On Epidemiology And Management Of Acute Colonic Pseudo Obstruction: Nationwide Clinical Data Research Network, Abhilash Perisetti Md, Hermant Goyal Md, Syed Mahanazuddin Phd, Mariajose Rojas Md, Mahesh Gajendran Md, Syed Ali Amir Sherazi Md, Shweta Gupta Md, Mbs, Ragesh Thandassery Md, Benjamin Tharian Md, Mrcp, Fracp, Neil Sharma Md

Other Specialties

Introduction Acute colonic pseudo-obstruction (ACPO), also known as Ogilvie’s syndrome, is characterized by massive acute dilatation of the large intestine without a mechanical cause. Early recognition to identify serious adverse events such as ischemia/ perforation is critical to reducing mortality rates. COVID-19 pandemic led to a significant shift of resources for the treatment of patients with reduced utilization of endoscopy and imaging, which could have impacted outcomes in patients with ACPO. In this study, we aim to identify the change in the clinical outcomes in ACPO patients in 2020.

Methods All adult patients with a new diagnosis of intestinal obstruction …

Contingency Management Implementation: Strengthening Mat In Primary Care, Angela Colistra Lpc, Phd, Ms, Sophia Harbove Ms, Ctp, Yamelisa Taveras Ma, Caadc, Ccs, Abby S. Letcher Md, Nyann Biery Ms

Department of Family Medicine

No abstract provided.

The Focused Rapid Assessment Process (Frap): Utilizing Geospatial Mapping And Rapid Qualitative Analysis To Impact Cancer Survivorship Disparities, Autumn Kieber-Emmons Md, Mph

Department of Family Medicine

No abstract provided.

A Case Of Abdominal Compartment Syndrome In Cardiac Surgery: A Rare, Potentially Fatal Complication., Michelle Chen, K. Gage Parr

Anesthesiology and Critical Care Medicine Faculty Posters and Presentations

No abstract provided.

Approaching Difficult Topics With Patients Panel Discussion, Lynn M. Wilson Do, Jeremy Fischer, S Carter, Rob Danoff

Department of Family Medicine

No abstract provided.

Facilitating Student Community Health Immersion During Covid-19 Crisis: The Promise Neighborhood Health Equity Fellowship And Bridging The Gaps Community Health Internship Collaboration., Abby S. Letcher Md, Hasshan Batts, Sirry Alang, Mary Ellen Miller

Department of Family Medicine

No abstract provided.