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Full-Text Articles in Medicine and Health Sciences
The Differentiation Of Hepatocyte-Like Cells From Monkey Embryonic Stem Cells., Xiaocui Ma, Jian Wu, M Zern
The Differentiation Of Hepatocyte-Like Cells From Monkey Embryonic Stem Cells., Xiaocui Ma, Jian Wu, M Zern
jian duan wu
Embryonic stem cells (ESC) hold great potential for the treatment of liver diseases. Here, we report the differentiation of rhesus macaque ESC along a hepatocyte lineage. The undifferentiated monkey ESC line, ORMES-6, was cultured in an optimal culture condition in an effort to differentiate them into hepatocyte-like cells in vitro. The functional efficacy of the differentiated hepatic cells was evaluated using RT-PCR for the expression of hepatocyte specific genes, and Western blot analysis and immunocytochemistry for hepatic proteins such as alpha-fetoprotein (AFP), albumin and alpha1-antitrypsin (alpha1-AT). Functional assays were performed using the periodic acid schiff (PAS) reaction and ELISA. The …
Evaluation Of Scrotal Masses, Jeffrey D. Tiemstra Md, S Kapoor
Evaluation Of Scrotal Masses, Jeffrey D. Tiemstra Md, S Kapoor
Jeffrey Tiemstra, MD, FAAFP
Mira V: An Integrated System For Minimally Invasive Robot-Assisted Lung Brachytherapy, A. Trejos, A. Lin, S. Mohan, H. Bassan, C. Edirisinghe, R. Patel, C. Lewis, E. Yu, A. Fenster, R. Malthaner
Mira V: An Integrated System For Minimally Invasive Robot-Assisted Lung Brachytherapy, A. Trejos, A. Lin, S. Mohan, H. Bassan, C. Edirisinghe, R. Patel, C. Lewis, E. Yu, A. Fenster, R. Malthaner
Edward Yu
An integrated system for minimally invasive robot-assisted image-guided lung brachytherapy has been developed. The system incorporates an experimental setup for accurate radioactive seed placement with commercially available dosimetry planning software. The end result is a complete system that allows planning and executing a brachytherapy procedure with increased accuracy. The results of the in vitro seed placement evaluation show that seed misplacement has a significant effect on the volume receiving more than 200% of the dose (V200), and the minimum dosage received by 90% of the volume (D90).
Use Of Bioluminescent Imaging To Assay The Transplantation Of Immortalized Human Fetal Hepatocytes Into Mice., Moon Seok Choi, Andreea M Catana, Jian Wu, Young Seok Kim, Sang Jeong Yoon, Alexander D Borowsky, Sanjiv S Gambhir, Sanjeev Gupta, Mark A Zern
Use Of Bioluminescent Imaging To Assay The Transplantation Of Immortalized Human Fetal Hepatocytes Into Mice., Moon Seok Choi, Andreea M Catana, Jian Wu, Young Seok Kim, Sang Jeong Yoon, Alexander D Borowsky, Sanjiv S Gambhir, Sanjeev Gupta, Mark A Zern
jian duan wu
Noninvasive serial monitoring of the fate of transplanted cells would be invaluable to evaluate the potential therapeutic use of human hepatocyte transplantation. Therefore, we assessed the feasibility of bioluminescent imaging using double or triple fusion lentiviral vectors in a NOD-SCID mouse model transplanted with immortalized human fetal hepatocytes. Lentiviral vectors driven by the CMV promoter were constructed carrying reporter genes: firefly luciferase and green fluorescence protein with or without herpes simplex virus type 1 thymidine kinase. Human fetal hepatocytes immortalized by telomerase reconstitution (FH-hTERT) were successfully transduced with either of these fusion vectors. Two million stably transduced cells selected by …