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Full-Text Articles in Medicine and Health Sciences
The Effects Of Age And Heterochromatin On Frequencies Of Acquired Chromosomal Aneuploidy In Uncultured Human Leukocytes, Noran Aboalela
The Effects Of Age And Heterochromatin On Frequencies Of Acquired Chromosomal Aneuploidy In Uncultured Human Leukocytes, Noran Aboalela
Theses and Dissertations
While age-related sex chromosomal aneuploidy is a well-characterized phenomenon, the relationship between autosomal loss and age remains unclear. The emergence of the specific and highly sensitive fluorescence in situ hybridization (FISH) technology has enabled investigators to study interphase cells, thereby overcoming problems inherent with the study of metaphase spreads for acquired aneuploidy assessment. Despite all the advantages of this technique, there are some limitations that could be misleading when scoring interphase autosomal aneuploidy. In this study we show that sex chromosomal hypoploidy is correlated with age. By using a twin study design, we evaluated Y chromosome hypoploidy frequencies and found …
Analyzing Dna Topology And Transcription In Salmonella Enterica Serovar Typhimurium During Dichotomous Growth, Betty M. Booker
Analyzing Dna Topology And Transcription In Salmonella Enterica Serovar Typhimurium During Dichotomous Growth, Betty M. Booker
All ETDs from UAB
The bacterial chromosome is dynamic. The principle goal of my research is to understand how DNA topology is altered by transcription in Salmonella enterica serovar Typhimurium LT2. Gamma delta-resolution requires two direct repeat Res sites to pair a plectonemic synapse. Previous work from our lab showed that the zones of high transcription inhibited gamma delta-resolution. Using phage λ recombineering methods, we have developed Salmonella strains to study ribosomal RNA operons, which are the most highly transcribed genes in bacteria. We propose a molecular model for how supercoiling generated by high levels of transcription modifies chromosome structure.