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Full-Text Articles in Medicine and Health Sciences
Immunologic Indicators Of Clinical Progression During Canine Leishmania Infantum Infection, Paola M. Boggiatto, Amanda E. Ramer-Tait, Erin E. Kramer, Kyle Metz, Katherine Gibson-Corley, Kathleen Mullin, Jesse M. Hosterner, Jack M. Gallup, Douglas E. Jones, Christine A. Petersen
Immunologic Indicators Of Clinical Progression During Canine Leishmania Infantum Infection, Paola M. Boggiatto, Amanda E. Ramer-Tait, Erin E. Kramer, Kyle Metz, Katherine Gibson-Corley, Kathleen Mullin, Jesse M. Hosterner, Jack M. Gallup, Douglas E. Jones, Christine A. Petersen
Amanda Ramer-Tait Publications
In both dogs and humans Leishmania infantum infection is more prevalent than disease, as infection often does not equate with clinical disease. Previous studies additively indicate that advanced clinical visceral leishmaniasis is characterized by increased production of anti-Leishmania antibodies, Leishmania-specific lymphoproliferative unresponsiveness, and decreased production of gamma interferon (IFN-γ) with a concomitant increase of interleukin-10 (IL-10). In order to differentiate infection versus progressive disease for better disease prognostication, we temporally evaluated humoral and cellular immunologic parameters of naturally infected dogs. The work presented here describes for the first time the temporal immune response to natural autochthonous L. …
Reduced Hamster Usage And Stress In Propagating Leishmania Chagasi Promastigotes Using Cryopreservation And Saphenous Vein Inoculation, Soi Meng Lei, Amanda E. Ramer-Tait, Rebecca R. Dahlin-Laborde, Kathleen Mullin, Jeffrey K. Beetham
Reduced Hamster Usage And Stress In Propagating Leishmania Chagasi Promastigotes Using Cryopreservation And Saphenous Vein Inoculation, Soi Meng Lei, Amanda E. Ramer-Tait, Rebecca R. Dahlin-Laborde, Kathleen Mullin, Jeffrey K. Beetham
Amanda Ramer-Tait Publications
Leishmania chagasi, a causal agent of visceral leishmaniasis, requires passage through lab animals such as hamsters to maintain its virulence. Hamster infection is typically accomplished via cardiac puncture or intraperitoneal injection, procedures accompanied by risks of increased animal stress and death. The use of the hamster model also necessitates a regular supply of infected animals, because L. chagasi parasites newly isolated from an infected hamster can be grown in culture for only several weeks before loss of function/phenotype occurs. In an effort to decrease animal usage and animal stress, experiments were performed to assess a more gentle inoculation procedure …