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Journal Articles: Biochemistry & Molecular Biology
Animals, Apoptosis, Apoptosis Regulatory Proteins, Cell Line, Tumor, DNA-Binding Proteins, Drug Interactions, Endoplasmic Reticulum, Fatty Acids, Monounsaturated, Fatty Liver, Hepatocytes, Humans, JNK Mitogen-Activated Protein Kinases, Membrane Proteins, Mice, Palmitates, Phosphorylation, Proto-Oncogene Proteins, RNA, Messenger, Stress, Physiological, Transcription Factor CHOP, Transcription Factors, Tumor Suppressor Proteins, Up-Regulation, bcl-2-Associated X Protein
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Full-Text Articles in Medicine and Health Sciences
Palmitoleate Attenuates Palmitate-Induced Bim And Puma Up-Regulation And Hepatocyte Lipoapoptosis., Yuko Akazawa, Sophie Cazanave, Justin L. Mott, Nafisa Elmi, Steven F. Bronk, Shigeru Kohno, Michael R. Charlton, Gregory J. Gores
Palmitoleate Attenuates Palmitate-Induced Bim And Puma Up-Regulation And Hepatocyte Lipoapoptosis., Yuko Akazawa, Sophie Cazanave, Justin L. Mott, Nafisa Elmi, Steven F. Bronk, Shigeru Kohno, Michael R. Charlton, Gregory J. Gores
Journal Articles: Biochemistry & Molecular Biology
BACKGROUND & AIMS: Saturated free fatty acids induce hepatocyte lipoapoptosis. This lipotoxicity involves an endoplasmic reticulum stress response, activation of JNK, and altered expression and function of Bcl-2 proteins. The mono-unsaturated free fatty acid palmitoleate is an adipose-derived lipokine which suppresses free fatty acid-mediated lipotoxicity by unclear mechanisms. Herein we examined the mechanisms responsible for cytoprotection.
METHODS: We employed isolated human and mouse primary hepatocytes, and the Huh-7 and Hep 3B cell lines for these studies. Cells were incubated in presence and absence of palmitate (16:0), stearate (18:0), and or palmitoleate (16:1, n-7).
RESULTS: Palmitoleate significantly reduced lipoapoptosis by palmitate …