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Full-Text Articles in Medicine and Health Sciences
Comparison Of Standard Agglutination Tests, Enzyme Immunoassay, And Coombs Gel Test Used In Laboratory Diagnosis Of Human Brucellosis, Emi̇ne Emel Koçman, Memnune Selda Erensoy, Meltem Taşbakan, Meltem Çi̇çekli̇oğlu
Comparison Of Standard Agglutination Tests, Enzyme Immunoassay, And Coombs Gel Test Used In Laboratory Diagnosis Of Human Brucellosis, Emi̇ne Emel Koçman, Memnune Selda Erensoy, Meltem Taşbakan, Meltem Çi̇çekli̇oğlu
Turkish Journal of Medical Sciences
Background/aim: It was aimed to evaluate the results of Rose Bengal (RB), ELISA total tests (IgM and IgG), and the Brucella Coombs gel test (BCGT), which are used as screening tests, with the combined results of a tube agglutination test (standard Wright test: SWT) and a tube agglutination test with antihuman globulin (AHG TAT). Materials and methods: Samples from 97 patients prediagnosed with brucellosis (age ≥18 years) were screened with RB, ELISA, and BCGT. SWT < 160 samples and RB-negative but ELISA- or BCGT-positive samples were tested by AHG TAT. SWT ≥ 160 or AHG TAT ≥ 160 was taken as reference. Results: Thirty-two of 56 RB-positive samples and one RB-negative but ELISA- and BCGT-positive sample were found to be ≥160 with SWT or AHG TAT. Sensitivity, specificity, accuracy, and agreement (kappa) values according to SWT ≥ 160 or AHG TAT ≥ 160 positivity were as follows, respectively: RB 96.9%, 62.5%, 74.2%, and 0.509; ELISA total 100%, 60.9%, 74.2%, and 0.515; BCGT test 97%, 70.3%, 79.4%, and 0.594. Conclusion: Sensitivities of the screening tests are similar, but positivities should be confirmed by more specific tests. Positive samples from screening tests should be tested with AHG if the SWT value is
Parvovirus B19 Seroprevalence In Turkish Blood Donors, Şeni̇z Göral, İdi̇l Yeni̇cesu, Gülendam Bozdayi, Aysu Duyan Çamurdan, Ayli̇n Altay Koçak
Parvovirus B19 Seroprevalence In Turkish Blood Donors, Şeni̇z Göral, İdi̇l Yeni̇cesu, Gülendam Bozdayi, Aysu Duyan Çamurdan, Ayli̇n Altay Koçak
Turkish Journal of Medical Sciences
Background/aim: Parvovirus risk in blood transfusion has become a popular research topic since there are limited data on parvovirus seroprevalence in blood donors in Turkey. The aim of this study was to investigate parvovirus seroprevalence in blood donors in Turkey. Materials and methods: Blood samples of 988 blood donors admitted to a university blood bank were obtained for parvovirus B19 IgM and IgG detection. The samples were analyzed using the ELISA method. Results: IgM positivity of 3.92% and IgG positivity of 58.9% was detected in the blood samples. Parvovirus IgM positivity was found to be the highest in the age …
Nitric Oxide In Cerebrospinal Fluid Of Central Nervous System Tuberculosis: Correlations With Culture, Antibody Response, And Cell Count, Kavitha Kumar, Prashant Giribhattanavar, Shripad Patil
Nitric Oxide In Cerebrospinal Fluid Of Central Nervous System Tuberculosis: Correlations With Culture, Antibody Response, And Cell Count, Kavitha Kumar, Prashant Giribhattanavar, Shripad Patil
Turkish Journal of Medical Sciences
Background/aim: The role of nitric oxide (NO) has been established in infection over the years. NO functions by inhibiting the growth of intracellular pathogens. The present study was undertaken to ascertain the role of NO in central nervous system (CNS) infection by Mycobacterium tuberculosis. Materials and methods: A total of 781 chronic meningitis cerebrospinal fluid (CSF) samples suspected of CNS tuberculosis (TB) were categorized based on M. tuberculosis culture positivity, anti-TB antibody response, and CSF cell count and were analyzed for NO. Results: We found that NO levels were positive in 10.88% of the CSF samples. Positivity for NO was …
Screening Of Immunocompromised Patients At Risk Of Strongyloidiasis In Western Turkey Using Elisa And Real-Time Pcr, Esra Erdem Kivrak, Esra Atalay Şahar, Hüseyi̇n Can, Mert Döşkaya, Mümtaz Yilmaz, Hüsnü Pullukçu, Ayşe Caner, Hüseyi̇n Töz, Adnan Yüksel Gürüz, Meltem Işikgöz Taşbakan
Screening Of Immunocompromised Patients At Risk Of Strongyloidiasis In Western Turkey Using Elisa And Real-Time Pcr, Esra Erdem Kivrak, Esra Atalay Şahar, Hüseyi̇n Can, Mert Döşkaya, Mümtaz Yilmaz, Hüsnü Pullukçu, Ayşe Caner, Hüseyi̇n Töz, Adnan Yüksel Gürüz, Meltem Işikgöz Taşbakan
Turkish Journal of Medical Sciences
Background/aim: Strongyloides stercoralis causes life-threatening hyperinfection or disseminated strongyloidiasis in immunocompromised patients such as HIV-positive, organ transplantation, and cancer patients. This study investigated the presence of strongyloidiasis in immunocompromised patients for the first time in Turkey. Materials and methods: Serum and stool samples were collected from 108 patients (25.9% of them were chronic renal failure and 74.1% were renal transplantation patients) who were admitted to Ege University Medical School in İzmir, located in western Turkey. Serum samples were analyzed by ELISA (DRG, Germany) and the presence of 18S rRNA gene of S. stercoralis was detected in stool samples by real-time …
A Study Of The Chikungunya Virus In Humans In Turkey, Tuğba Atalay, Sedat Kaygusuz, Ahmet Kürşat Azkur
A Study Of The Chikungunya Virus In Humans In Turkey, Tuğba Atalay, Sedat Kaygusuz, Ahmet Kürşat Azkur
Turkish Journal of Medical Sciences
Background/aim: The chikungunya virus (CHIKV) is a mosquito-borne disease and has recently been causing explosive outbreaks. The CHIKV has spread throughout all continents. Although the first chikungunya case imported from India to Turkey was reported in 2012, there is no detailed epidemiologic study in Turkey yet. The aim of this study was to investigate the seroprevalence of the CHIKV in Turkey. Materials and methods: ELISA was used to screen 500 random serum samples of healthy people collected from Kırıkkale, which is located in central Anatolia in Turkey. The results were verified by indirect immunofluorescence test (IIFT). Results: The results showed …
Comparison Of Microscopy, Elisa, And Real-Time Pcr For Detectionof Giardia Intestinalis In Human Stool Specimens, Yunus Emre Beyhan, Zeynep Taş Cengi̇z
Comparison Of Microscopy, Elisa, And Real-Time Pcr For Detectionof Giardia Intestinalis In Human Stool Specimens, Yunus Emre Beyhan, Zeynep Taş Cengi̇z
Turkish Journal of Medical Sciences
Background/aim: This study included patients who had digestive system complaints between August 2015 and October 2015. The research was designed to compare conventional microscopy with an antigen detection ELISA kit and the TaqMan-based real-time PCR (RT-PCR) technique for detection of Giardia intestinalis in human stool specimens. Materials and methods: Samples were concentrated by formalin-ether sedimentation technique and microscopic examinations were carried out on wet mount slides. A commercially available ELISA kit (Giardia CELISA, Cellabs, Brookvale, Australia) was used for immunoassay. DNA was extracted from fecal samples of about 200 mg using the QIAamp Fast DNA Stool Mini Kit (QIAGEN, Hilden, …
Quantitative Comparison Of Immunohistochemical And Pcr Analysis Of Midkine Expression In Breast Cancer Types And Serum Midkine Level, Hülya Çeti̇n Sorkun, Meti̇n Akbulut, Yaşar Enli̇, Emre Tepeli̇, Sevgi̇ Özkan, Ergün Erdem
Quantitative Comparison Of Immunohistochemical And Pcr Analysis Of Midkine Expression In Breast Cancer Types And Serum Midkine Level, Hülya Çeti̇n Sorkun, Meti̇n Akbulut, Yaşar Enli̇, Emre Tepeli̇, Sevgi̇ Özkan, Ergün Erdem
Turkish Journal of Medical Sciences
Background/aim: Midkine (MK), a heparin-binding growth factor, has an important role in cancer progression. The aim of this study was to determine MK expression in breast tissue and the preoperative and postoperative serum levels of patients with breast cancer. Materials and methods: Sixty-one patients with breast cancer participated in our study. The MK serum levels were measured pre- and postoperatively for these patients. We also analyzed breast tissues of the 61 patients immunohistochemically. We examined serum midkine levels in 49 healthy volunteers. Results: MK expression was observed in 44 (72.1%) of 61 breast cancer patients. In breast cancer patients the …
Evaluation Of 4 Methods For The Serological Diagnosis Of EpsteinBarr Virus Infection Using An Immunofluorescence Assay As The Reference Method, Mücahi̇de Esra Koçoğlu, Teki̇n Taş, Firat Zafer Mengeloğlu, Şeyda Özsoy, Özlem Bucak
Evaluation Of 4 Methods For The Serological Diagnosis Of EpsteinBarr Virus Infection Using An Immunofluorescence Assay As The Reference Method, Mücahi̇de Esra Koçoğlu, Teki̇n Taş, Firat Zafer Mengeloğlu, Şeyda Özsoy, Özlem Bucak
Turkish Journal of Medical Sciences
Tests specific for VCA IgM, VCA IgG, and EBNA IgG are used to diagnose Epstein-Barr virus (EBV) infections and interpret disease status. The immunofluorescence assay (IFA) is accepted as the gold standard test. The purpose of this study was to evaluate the performance of 4 methods in comparison with IFA. Materials and methods: In total, 101 serum samples were obtained from clinically suspected cases of EBV infection between May 2010 and May 2012 and evaluated by IFA. All serum samples were analyzed by an immunoblot assay, enzyme-linked fluorescent assay (ELFA), enzyme immunoassay (EIA), and immunochromatographic assay (ICA). Results: ELFA and …
Possible Role Of Toxoplasmosis In Patients With First-Episode Schizophrenia*, Mehmet Tanyüksel, Özcan Uzun, Engi̇n Araz, Özgür Koru, Cahi̇t Babür
Possible Role Of Toxoplasmosis In Patients With First-Episode Schizophrenia*, Mehmet Tanyüksel, Özcan Uzun, Engi̇n Araz, Özgür Koru, Cahi̇t Babür
Turkish Journal of Medical Sciences
To determine the possible relationship between toxoplasmosis and patients with first-episode schizophrenia (FES). Materials and methods: Seventy-three subjects with FES (15-54 years old; mean: 23.4 years) and 40 healthy individuals (20-54 years old; mean: 30.3 years) were enrolled in the study. Most of the FES patients (90.4%) and the control individuals (95.0%) were male. Specific IgG and IgM antibodies to Toxoplasma gondii were investigated by enzyme-linked immunosorbent assay (ELISA), and the total amount of antibodies was investigated with the Sabin-Feldman dye test (SFDT). Results: Compared to the control individuals, study subjects had significantly more toxoplasmosis-like symptoms and more cats in …
Evaluation Of Serological Tests For The Diagnosis Of Visceral Leishmaniasis, Selçuk Kiliç, Ayşegül Taylan Özkan, Cahi̇t Babür, Gönül Tanir, Henk Dfh Schallig
Evaluation Of Serological Tests For The Diagnosis Of Visceral Leishmaniasis, Selçuk Kiliç, Ayşegül Taylan Özkan, Cahi̇t Babür, Gönül Tanir, Henk Dfh Schallig
Turkish Journal of Medical Sciences
Aim: Visceral leishmaniasis (VL) is a zoonosis that affects both animals and man. VL is seen sporadically in the Aegean, Mediterranean, Black Sea and central Anatolian regions of Turkey. The aim of this study was to evaluate serological tests for the diagnosis of VL. Materials and Methods: The performance of two agglutination tests based on freeze-dried whole promastigote Leishmania antigen, i.e. Fast Agglutination Screening Test (FAST) and Direct Agglutination Test (DAT), an Indirect Immunofluorescent Antibody Test (IFAT), and an Enzyme-Linked Immunosorbent Assay (ELISA) using soluble antigen were compared to parasitological diagnosis in the serum specimens of 59 patients with the …
Development Of A Highly Sensitive Elisa For Quantification Of Hepatitis B Virus (Hbv) Surface Antigen (Hbsag), Resul Karakuş, Lati̇fe Arzu Aral, Bi̇lkay Baştürk, Cemaletti̇n Aybay
Development Of A Highly Sensitive Elisa For Quantification Of Hepatitis B Virus (Hbv) Surface Antigen (Hbsag), Resul Karakuş, Lati̇fe Arzu Aral, Bi̇lkay Baştürk, Cemaletti̇n Aybay
Turkish Journal of Medical Sciences
Background: Hepatitis B Virus (HBV) infection is of global importance and many studies are aimed to develop more sensitive diagnostic tools and improve therapeutic options. HBV infection is diagnosed and followed-up basically by serological studies, among which the enzyme-linked immunosorbent assay (ELISA) is widely used. Aim: To develop a sensitive HBsAg ELISA for both research and diagnostic purposes. Materials and Methods: A hybridoma (clone 4D1) secreting anti-HBs IgG2a monoclonal antibody (mAb) was used for the establishment of our in-house ELISA. Tests were performed for both cell culture medium and human sera. The hepatoma cell lines of human origin PLC/PRF/5 and …
Comparison Of The Diagnostic Value Of The Standard Tube Agglutination Test And The Elisa Igg And Igm In Patients With Brucellosis, Mustafa Ertek, Hali̇l Yazgi, Zülal Özkurt, Ahmet Ayyildiz, Mehmet Parlak
Comparison Of The Diagnostic Value Of The Standard Tube Agglutination Test And The Elisa Igg And Igm In Patients With Brucellosis, Mustafa Ertek, Hali̇l Yazgi, Zülal Özkurt, Ahmet Ayyildiz, Mehmet Parlak
Turkish Journal of Medical Sciences
Background and Aims: Brucellosis is endemic in Turkey. Since it affects many organs and the symptoms are non-specific, the diagnosis by clinical findings is difficult and may be easily missed. Many serological tests have been used for the diagnosis of human brucellosis. This study compared the diagnostic value of the Brucella standard tube agglutination test (SAT) with that of ELISA (Brucella specific IgG and IgM) tests in patients with Brucella bacteremia. Patients and Methods: Thirty-two patients with brucellosis who had positive blood and/or bone-marrow cultures for Brucella species, and 20 healthy individuals as controls were included in the study. Results: …
Development Of Elisa Systems For Measurement Of Human Tumor Necrosis Factor-Alpha (Tnf-\Alpha), Ayşegül Yücel, Cemaletti̇n Aybay
Development Of Elisa Systems For Measurement Of Human Tumor Necrosis Factor-Alpha (Tnf-\Alpha), Ayşegül Yücel, Cemaletti̇n Aybay
Turkish Journal of Medical Sciences
The aim of this study was to develop a human tumor necrosis factor alpha (hTNF-\alpha) ELISA system because specific and sensitive measurement of low levels of circulating TNF-a is very important for enlightening the immunopathologial mechanisms associated with TNF-\alpha. Monoclonal antibodies 6A4c and 8A6 were produced against hTNF-\alpha and were used as the capture antibody and the tracer antibody respectively for the first hTNF-\alpha ELISA system (6A4c/biotin-8A6). Murine polyclonal IgG was used as the tracer antibody in the second hTNF-\alpha ELISA system (6A4c/biotin-polyclonal IgG). Both systems could detect both recombinant hTNF-\alpha and native hTNF-\alpha. The detection limits defined as minimal …
Serological Evaluation Of Hsv-1 And Hsv-2 Infection In Pregnancy, Ni̇zami̇ Duran
Serological Evaluation Of Hsv-1 And Hsv-2 Infection In Pregnancy, Ni̇zami̇ Duran
Turkish Journal of Medical Sciences
We performed a serological evaluation of herpes simplex virus (HSV) infections at the time of labor. In order to determine the seroprevalence of asymptomatic HSV infections in pregnant women, blood samples were collected for antibody measurement from 130 randomly selected pregnant women. Presence of HSV IgG and IgM antibodies in the serum samples was tested by ELISA. The prevalence of HSV-1 gG1 antibodies was 90% (117/130), that of HSV-2 gG2 antibodies was 63.1% (82/130) and that of HSV-2 IgM antibodies was 13.8% (18/130). Eight (44.4%) of the pregnant women with HSV-2 IgM were at the same time found to have …
Measurement Of Urine Albumin Levels With A Monoclonal Antibody Based In-House Elisa, Cemaletti̇n Aybay, Resul Karakuş
Measurement Of Urine Albumin Levels With A Monoclonal Antibody Based In-House Elisa, Cemaletti̇n Aybay, Resul Karakuş
Turkish Journal of Medical Sciences
Microalbuminuria is considered to be an early indicator of diabetic nephropathy. We have previously reported the establishment of A-HAlb/98 hybridoma secreting monoclonal antibody that specifically recognizes human serum albumin (HSA). In this report, an in-house enzyme-linked immunosorbent assay (ELISA) was developed using this antibody for the measurement of urinary albumin (UA) and UA was examined in 24 different samples. A-HAlb/98 was used as the capture antibody by immobilizing onto microtitre plates in ELISA format. HSA-specific polyclonal rabbit immunoglobulin was labelled with biotin and then used as the tracer antibody. The dynamic assay range of ELISA for albumin was established to …
Development Of A Diagnostic And Screening Elisa System For Measuring Tetanus Antitoxoid Levels, Cemaletti̇n Aybay, Resul Karakuş, Ahmet Gökhan Gündoğdu
Development Of A Diagnostic And Screening Elisa System For Measuring Tetanus Antitoxoid Levels, Cemaletti̇n Aybay, Resul Karakuş, Ahmet Gökhan Gündoğdu
Turkish Journal of Medical Sciences
Tetanus is a vaccine-preventable disease of global importance. When acquired it has a high mortality rate. Screenings of special subpopulations such as women of childbearing age provide important clues about tetanus vaccination coverage, and tools for mass protective measures, e.g, protective measures for neonatal tetanus. In addition to its reliability and sensitivity, the main advantage of a mass-screening tool should be its cost-effectiveness. We aimed to develop a cost-effective and sensitive enzyme-linked immunosorbent assay (ELISA) that can be used for diagnostic research as well as mass screening purposes for antitetanus antibodies. Our in-house antitetanus ELISA was validated and tested for …
Sandwich-Type Enzyme Immunoassay For Amyloid A4 Protein In Cerebrospinal Fluid From Patients With Head Trauma, İbrahi̇m Pi̇ri̇m
Sandwich-Type Enzyme Immunoassay For Amyloid A4 Protein In Cerebrospinal Fluid From Patients With Head Trauma, İbrahi̇m Pi̇ri̇m
Turkish Journal of Medical Sciences
The aim of this study was to design a simple method for detecting the amyloid A4 protein in the cerebrospinal fluid (CSF) of patients who had severe brain trauma caused by traffic or other accidents and to evaluate its diagnostic and prognostic value. For this purpose, a sandwich type ELISA assay was used. A4, a 4kDa protein of 39-43 amino acids, is a metabolic product of a membrane-spanning, 695-770 aa, precursor molecule, the b-amyloid precursor protein (b-APP). The polyclonal rabbit antibody against synthetic A4 protein (1-40 residues) was used as an immobilized antibody. A mouse monoclonal antibody against synthetic A4 …
Production Of Monoclonal Antibody Specific For Human Hemoglobin, Selma Öztürk, Beyazit Çirakoğlu
Production Of Monoclonal Antibody Specific For Human Hemoglobin, Selma Öztürk, Beyazit Çirakoğlu
Turkish Journal of Medical Sciences
Hemoglobin is not normally present in sputum, urine or feces except in minute amounts. Demonstration of the presence of hemoglobin in these materials is an important diagnostic clue that usually implies bleeding within the respiratory, urinary or gastroentestinal tract, respectively. In this work, a hybridoma line secreting monoclonal antibody (Mab) highly specific for human hemoglobin A (hHbA) was generated by fusion of immunized BALB/c mouse splenic lymphocytes with non-secreting mouse myeloma cells followed by selection in hypoxanthine-aminopterin-thymidine (HAT) medium and the screening of culture fluids for hHb A affinity in ELISA. The Mab produced by MAM 2/3B6 is highly specific …
Value Of Anti-Kp 90 Lmmunoglobulin A (Lga)Antibodies In Diagnosis Of Active Tuberculosis: Apreliminary Report, Sevtap Arikan, Serdar Tuncer, Dürdal Us, Serhat Ünal, Şemsettin Ustaçelebi̇
Value Of Anti-Kp 90 Lmmunoglobulin A (Lga)Antibodies In Diagnosis Of Active Tuberculosis: Apreliminary Report, Sevtap Arikan, Serdar Tuncer, Dürdal Us, Serhat Ünal, Şemsettin Ustaçelebi̇
Turkish Journal of Medical Sciences
The lack of a rapid and reliable method for diagnosis of active tuberculosis necessitated the development of new laboratory techniques. Diagnostic value of a re-cently developed ELISA for the detection of lgA antibodies to Kp 90 antigen of Mycobacterium tuberculosis was evaluated in this study and the results were compared with the conventional methods and polymerase chain reaction (PCR). Fifty six sera and body fluid (pleural, peritoneal, pericardial) samples from 23 patients with ac-tive tuberculosis and 26 control subjects were included in the study. Out of 28 samples obtained from the patients, 22 (79%) yielded positive result for lgA. Comparison …