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Articles 1 - 6 of 6
Full-Text Articles in Medicine and Health Sciences
Role Of Macrophage Apoptosis In Atherosclerosis., June Liu
Role Of Macrophage Apoptosis In Atherosclerosis., June Liu
Electronic Theses and Dissertations
The presence of apoptotic cells in atherosclerotic lesions has been broadly reported in the past ten years. The majority of these apoptotic cells are macrophages. However, the pathogenic role of macrophage apoptosis in the development of atherosclerosis remains to be elucidated. Elevated expression of Bax, one of the pivotal pro-apoptotic proteins of the Bcl-2 family, has been found in human atherosclerotic plaques. Activation of Bax also occurs in free cholesterol-loaded and oxysterol treated mouse macrophages. In this study, we evaluated the influence of Bax deficiency on apoptosis in macrophage-like P388D1 cells by using small interfering RNA (siRNA) to suppress Bax …
Degradation Of 23s Rrna In Azithromycin-Treated Ribonuclease Mutants Of Escherichia Coli., Jessica A. Silvers
Degradation Of 23s Rrna In Azithromycin-Treated Ribonuclease Mutants Of Escherichia Coli., Jessica A. Silvers
Electronic Theses and Dissertations
Azithromycin, a macrolide antibiotic, specifically binds to the 50S ribosomal subunit of bacterial ribosomes and inhibits translation. Azithromycin also prevents 50S ribosomal subunit assembly by binding to a 50S ribosomal subunit precursor particle. When exposed to azithromycin, several ribonucleases in wild-type Escherichia coli cells degrade antibiotic-bound 50S precursor particles. Presumably, cells expressing one or more mutated ribonucleases will degrade the antibiotic-bound precursor less efficiently, resulting in increased sensitivity to the antibiotic. To test this, eight ribonucleaseûdeficient strains of Escherichia coli were grown in the presence or absence of azithromycin. Cell viability, growth rates, and protein synthesis rates were measured. Degradation …
Dose-Dependent Effects Of Salmon Calcitonin On Bone Turnover In Ovariectomized Rats., Beatrice H. Owens
Dose-Dependent Effects Of Salmon Calcitonin On Bone Turnover In Ovariectomized Rats., Beatrice H. Owens
Electronic Theses and Dissertations
In the United States, osteoporosis results in about 1.5 million annual fractures, costing approximately $15 billion. Calcitonin is safe and effective in slowing osteoporotic bone loss, but its effect is transient. The current studies were designed to explore the dose-dependent effects of salmon calcitonin on bone turnover in ovariectomized rats and to determine if the decrease in therapeutic effectiveness of calcitonin demonstrated over time with higher doses is due to oversuppression of bone turnover. Doses of 5, 15, & 50 IU/kg BW/day of calcitonin were compared to placebo in 12-week-old ovariectomized and sham-ovariectomized Sprague-Dawley rats for 24 weeks. The spinal …
Papain: A Novel Urine Adulterant., David Burrows
Papain: A Novel Urine Adulterant., David Burrows
Electronic Theses and Dissertations
The estimated number of employees in the United Stated screened annually for illicit drugs is approximately 20 million, with marijuana being the most frequently abused drug. Urine adulterants provide an opportunity for illicit drug users to obtain a false negative result on commonly used primary drug screening methods such as the Fluorescence Polarized Immunoassay (FPIA) technique. Typical chemical adulterants such as nitrites are easily detected or render the urine specimen invalid as defined in the proposed federal guidelines for specimen validity testing based on creatinine, specific gravity and pH. Papain is a cysteine protease with intrinsic ester hydrolysis capability. The …
Nongenomic Effects Of Estrogens On Epithelial Chloride Secretion., Sabyasachi Moulik
Nongenomic Effects Of Estrogens On Epithelial Chloride Secretion., Sabyasachi Moulik
Electronic Theses and Dissertations
The human colonic cell line T84, a model for studying epithelial chloride secretion and cystic fibrosis chloride channel (CFTR) function, was used to investigate the regulatory role of estrogens in transepithelial ion transport. Estrogens and other steroid hormones do not stimulate chloride secretion by themselves. However, 17 β-estradiol (17β-E2) rapidly (within seconds to minutes) potentiates carbachol- and thapsigargin-stimulated chloride secretion measured as short circuit current in voltage-clamped T84 monolayer cultures. The cholinergic agonist carbachol and the SR Ca2+ ATPase inhibitor thapsigargin stimulate chloride secretion by elevating intracellular calcium. 17α-estradiol, a stereoisomer that does not activate nuclear estrogen receptors, is …
The Role Of Osteopontin In Vascular Remodeling, Daniel Myers
The Role Of Osteopontin In Vascular Remodeling, Daniel Myers
Electronic Theses and Dissertations
Chronic obstructive vascular disease is a major contributor to mortality in developed nations. The pathology typically involves neointima formation accompanied by vascular remodeling in the presence of inflammation. The disease can be modeled in animals with the response to a direct injury of the vasculature. This text describes a project examining the role of osteopontin (OPN) in vascular remodeling. The projectis goal was to mechanistically describe the contribution of OPN to the vascular injury response. The hypothesis that the presence of OPN affects the formation of neointima and constrictive remodeling associated with vascular injury was addressed using the strategy of …