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Full-Text Articles in Medicine and Health Sciences
The Parkinson's Disease Protein Α-Synuclein Disrupts Cellular Rab Homeostasis, Aaron D. Gitler, Brooke J. Bevis, James Shorter, Katherine E. Strathearn, Shusei Hamamichi, Linhui Julie Su, Kim A. Caldwell, Guy A. Caldwell, Jean-Christophe Rochet, J. Michael Mccaffrey, Charles Barlowe, Susan Lindquist
The Parkinson's Disease Protein Α-Synuclein Disrupts Cellular Rab Homeostasis, Aaron D. Gitler, Brooke J. Bevis, James Shorter, Katherine E. Strathearn, Shusei Hamamichi, Linhui Julie Su, Kim A. Caldwell, Guy A. Caldwell, Jean-Christophe Rochet, J. Michael Mccaffrey, Charles Barlowe, Susan Lindquist
Dartmouth Scholarship
α-Synuclein (α-syn), a protein of unknown function, is the most abundant protein in Lewy bodies, the histological hallmark of Parkinson's disease (PD). In yeast α-syn inhibits endoplasmic reticulum (ER)-to-Golgi (ER→Golgi) vesicle trafficking, which is rescued by overexpression of a Rab GTPase that regulates ER→Golgi trafficking. The homologous Rab1 rescues α-syn toxicity in dopaminergic neuronal models of PD. Here we investigate this conserved feature of α-syn pathobiology. In a cell-free system with purified transport factors α-syn inhibited ER→Golgi trafficking in an α-syn dose-dependent manner. Vesicles budded efficiently from the ER, but their docking or fusion to Golgi membranes was inhibited. Thus, …
The Bile Response Repressor Brer Regulates Expression Of The Vibrio Cholerae Breab Efflux System Operon, Francisca A. Cerda-Maira, Carol S. Ringelberg, Ronald K. Taylor
The Bile Response Repressor Brer Regulates Expression Of The Vibrio Cholerae Breab Efflux System Operon, Francisca A. Cerda-Maira, Carol S. Ringelberg, Ronald K. Taylor
Dartmouth Scholarship
Enteric pathogens have developed several resistance mechanisms to survive the antimicrobial action of bile. We investigated the transcriptional profile of Vibrio cholerae O1 El Tor strain C6706 under virulence gene-inducing conditions in the presence and absence of bile. Microarray analysis revealed that the expression of 119 genes was affected by bile. The mRNA levels of genes encoding proteins involved in transport were increased in the presence of bile, whereas the mRNA levels of genes encoding proteins involved in pathogenesis and chemotaxis were decreased. This study identified genes encoding transcriptional regulators from the TetR family (vexR and breR) and …