Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Adult; article; B lymphocyte; binding site; blood cell; blood volume; cell lineage; controlled study; erythrocyte; gene control; gene expression; gene expression profiling; gene identification; genetic variability; granulocyte; hematopoietic cell; human; human cell; human experiment; male; normal human; quantitative analysis; reverse transcription polymerase chain reaction; T lymphocyte; transgene (1)
- Alpha-2-antiplasmin (1)
- Blood platelets (1)
- Cardeza Foundation for Hematologic Research (1)
- Cerebrospinal fluid (1)
Articles 1 - 3 of 3
Full-Text Articles in Medicine and Health Sciences
Fluorescent Peptide For Detecting Factor Xiiia Activity And Fibrin In Whole Blood Clots Forming Under Flow, Yue Liu, Jennifer Crossen, Timothy J. Stalker, Scott L. Diamond
Fluorescent Peptide For Detecting Factor Xiiia Activity And Fibrin In Whole Blood Clots Forming Under Flow, Yue Liu, Jennifer Crossen, Timothy J. Stalker, Scott L. Diamond
Cardeza Foundation for Hematologic Research
Background
During clotting, thrombin generates fibrin monomers and activates plasma-derived transglutaminase factor (F) XIIIa; collagen and thrombin-activated platelets offer thrombin-independent cellular FXIIIa (cFXIIIa) for clotting. Detecting fibrin on collagen and tissue factor surfaces in whole blood clotting typically uses complex reagents like fluorescent fibrinogen or antifibrin antibody.
Objectives
We want to test whether the peptide using the α2- antiplasmin crosslinking mechanism by FXIIIa is a useful tool in both monitoring FXIIIa activity, and visualize and monitor fibrin formation, deposition, and extent of crosslinking within fibrin structures in whole blood clots formed under flow.
Methods
We tested a fluorescent peptide derived …
Murine Mpdz-Linked Hydrocephalus Is Caused By Hyperpermeability Of The Choroid Plexus., Junning Yang, Claire Simonneau, Robert Kilker, Laura Oakley, Matthew D. Byrne, Zuzana Nichtova, Ioana Stefanescu, Fnu Pardeep-Kumar, Sushil Tripathi, Eric Londin, Pascale Saugier-Veber, Belinda Willard, Mathew Thakur, Stephen Pickup, Hiroshi Ishikawa, Horst Schroten, Richard Smeyne, Arie Horowitz
Murine Mpdz-Linked Hydrocephalus Is Caused By Hyperpermeability Of The Choroid Plexus., Junning Yang, Claire Simonneau, Robert Kilker, Laura Oakley, Matthew D. Byrne, Zuzana Nichtova, Ioana Stefanescu, Fnu Pardeep-Kumar, Sushil Tripathi, Eric Londin, Pascale Saugier-Veber, Belinda Willard, Mathew Thakur, Stephen Pickup, Hiroshi Ishikawa, Horst Schroten, Richard Smeyne, Arie Horowitz
Cardeza Foundation for Hematologic Research
Though congenital hydrocephalus is heritable, it has been linked only to eight genes, one of which is MPDZ Humans and mice that carry a truncated version of MPDZ incur severe hydrocephalus resulting in acute morbidity and lethality. We show by magnetic resonance imaging that contrast medium penetrates into the brain ventricles of mice carrying a Mpdz loss-of-function mutation, whereas none is detected in the ventricles of normal mice, implying that the permeability of the choroid plexus epithelial cell monolayer is abnormally high. Comparative proteomic analysis of the cerebrospinal fluid of normal and hydrocephalic mice revealed up to a 53-fold increase …
Microrna Expression Differences In Human Hematopoietic Cell Lineages Enable Regulated Transgene Expression., Raúl Teruel Montoya, Xianguo Kong, Shaji Abraham, Lin Ma, Satya P Kunapuli, Michael Holinstat, Chad A Shaw, Steven E. Mckenzie, Leonard Edelstein, Paul Bray
Microrna Expression Differences In Human Hematopoietic Cell Lineages Enable Regulated Transgene Expression., Raúl Teruel Montoya, Xianguo Kong, Shaji Abraham, Lin Ma, Satya P Kunapuli, Michael Holinstat, Chad A Shaw, Steven E. Mckenzie, Leonard Edelstein, Paul Bray
Cardeza Foundation for Hematologic Research
Blood microRNA (miRNA) levels have been associated with and shown to participate in disease pathophysiology. However, the hematopoietic cell of origin of blood miRNAs and the individual blood cell miRNA profiles are poorly understood. We report the miRNA content of highly purified normal hematopoietic cells from the same individuals. Although T-cells, B-cells and granulocytes had the highest miRNA content per cell, erythrocytes contributed more cellular miRNA to the blood, followed by granulocytes and platelets. miRNA profiling revealed different patterns and different expression levels of miRNA specific for each lineage. miR-30c-5p was determined to be an appropriate reference normalizer for cross-cell …