Medicine and Health Sciences Commons^™

Modulation Of The Cardiomyocyte Hypertrophic Responses To Endothelin-1 By Adipocytes, Suresh Chandra Bairwa

Electronic Thesis and Dissertation Repository

Leptin and adiponectin are the adipokines that are shown to exert pro-hypertrophic and anti-hypertrophic effects respectively, in cardiomyocytes. We sought to determine the direct interaction between the adipocytes and cardiomyocytes during endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy and determine the role of leptin and adiponectin.

The adipose tissue conditioned medium (ACM) inhibited the ET-1-induced hypertrophy in a concentration-dependent manner and this anti-hypertrophic effect was more potent or reversed in the presence of leptin receptor antagonist (LRA; 0.1 nM) or adiponectin receptor-1 antibody (ARA; 100 ng/mL), respectively. ACM from heart failure rats induced by coronary artery ligation or obese rats did not mitigate …

Fully Automated Segmentation And Quantification Of Abdominal Adipose Tissue Compartments In Mouse Mri, Colin M. Mccurdy

Electronic Thesis and Dissertation Repository

Obesity currently affects 25% of Canadians and is strongly associated with many diseases including diabetes, cardiovascular disease, and cancer. However, only Intra-Abdominal Adipose Tissue (IAAT) is an important predictor of obesity associated disease and mortality. Magnetic Resonance Imaging (MRI) is an effective modality for imaging fat and methods have been developed to automate segmentation of IAAT. Currently existing techniques for automated segmentation in mice require acquisition using high magnetic field MRI equipment and use image acquisition techniques with low precision for fat quantification. We demonstrate a new fully automated technique for fat quantification in clinical strength mouse MRI through adaptation …