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Quantitative Analysis Of Relaxation Rate Dependence On Interecho Time In Maga-Expressing, Iron-Labeled Cells, Casey Yesol Lee
Quantitative Analysis Of Relaxation Rate Dependence On Interecho Time In Maga-Expressing, Iron-Labeled Cells, Casey Yesol Lee
Electronic Thesis and Dissertation Repository
Reporter gene-based methods of labeling cells with iron is an emerging method of providing magnetic resonance imaging (MRI) contrast for long-term cell tracking and monitoring of cellular activities. This thesis investigates 9.4 T NMR properties of mammalian cells over-expressing a magnetotactic bacterial putative iron transport gene, MagA, and the associated untransfected parental cells. Cells were cultured in medium alone or supplemented with 250 μM ferric nitrate. Using the Carr-Purcell-Meiboom-Gill sequence, the relationship between R2 and interecho time was analyzed for each of the cell types using a model based on water diffusion in weak magnetic field inhomogeneities (Jensen and Chandra, …
Mri Relaxation Rates: A Quantitative Approach To Track Tumour Cells Expressing Maga, Anindita Sengupta
Mri Relaxation Rates: A Quantitative Approach To Track Tumour Cells Expressing Maga, Anindita Sengupta
Electronic Thesis and Dissertation Repository
Using magnetic resonance imaging, relaxation rate measurements were
performed in cancer cells overexpressing a magnetotactic bacterial gene, MagA.
Measurements of magnetic resonance relaxation rates in this expression
system is important for optimizing cell detection and specificity, for developing
quantification methods, and for refinement of gene-based iron contrast using
magnetosome associated genes. We measured the total transverse
relaxation rate (R2*), its irreversible and reversible components (R2 and R2′,
respectively) and the longitudinal relaxation rate (R1) in MDA-MB-435 tumor cells.
Clonal lines overexpressing MagA were cultured in the presence and absence of
iron supplementation, and mounted in a …