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Full-Text Articles in Medicine and Health Sciences
Influence Of Polycaprolactone And Basic Fibroblast Growth Factor On Gingival Fibroblasts, Sarah Michelsons
Influence Of Polycaprolactone And Basic Fibroblast Growth Factor On Gingival Fibroblasts, Sarah Michelsons
Electronic Thesis and Dissertation Repository
Guided tissue regeneration (GTR) to regenerate periodontal tissue involves placement of a cell-occlusive barrier membrane functionally excluding the gingiva and associated oral epithelium from the periodontal defect. Gingival connective tissue (CT) contains a rich vascular plexus and is a source of progenitor cells which could contribute to periodontal regeneration. We propose the use of a novel biodegradable and bioactive electrospun fibrous polycaprolactone (PCL) scaffold loaded with microspheres releasing basic fibroblast growth factor (bFGF) to promote gingival CT growth while maintaining a barrier to the oral epithelium. Scaffolds supported human gingival fibroblast proliferation and mesenchymal cell infiltration in a bFGF dose …
Tak1 Mediates Tgf Beta-1 Responses In Gingival Fibroblasts, Hanna Kuk
Tak1 Mediates Tgf Beta-1 Responses In Gingival Fibroblasts, Hanna Kuk
Electronic Thesis and Dissertation Repository
In fibroblasts, transforming growth factor β-1 (TGFβ1) signals via canonical and non-canonical pathways to promote wound healing or hyper-contractile responses resulting in scars. The oral cavity however does not scar and fibrotic responses such as gingival hyperplasia are characterized by a hyper-proliferative response. The involvement of non-canonical, TGFβ-activated kinase-1 (TAK1) - mediated TGFβ1 signaling in gingival fibroblasts has not been previously examined. Here I show that TAK1 selective inhibitor (5Z)-7-Oxozeaenol blocks TGFβ1-induced expression of wound healing and fibrotic marker CCN2 (connective tissue growth factor) in gingival fibroblasts. Genome-wide expression profiling revealed that essentially all TGFβ1 induced genes were in fact …