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Full-Text Articles in Medicine and Health Sciences
Temporal Changes In The Levels Of Gamma-Aminobutyric Acid In The Brain Of The Cockroach, Leucophaea Maderae, Jonathan Newton
Temporal Changes In The Levels Of Gamma-Aminobutyric Acid In The Brain Of The Cockroach, Leucophaea Maderae, Jonathan Newton
Masters Theses & Specialist Projects
Circadian rhythms have been shown to exist in all studied species and play an important role in their survival. The cockroach is an excellent model organism in which to study circadian systems because of the wealth of background information regarding both its anatomy and circadian system. Clock activity measured electrophysiologically in the cockroach system is 180 degrees out of phase with locomotor activity, with the maximum neural output occurring in the morning, twelve hours prior to the onset of locomotor activity. This inverse relationship of locomotor and neural activity suggests that the clock output may be inhibitory. In vertebrates, the …
Site Directed Mutagenesis Of A Putative Protease Cleavage Site Within The 105 Kilodation Protein Of Southern Bean Mosaic Virus, Jingyu Shi
Masters Theses & Specialist Projects
There are four open reading frames (ORF) in three positive-sense translational phases of Southern Bean Mosaic Virus (SBMV). ORF2 codes for a 105 KDa polyprotein which is proposed to be autocatalytically cleaved into smaller functional proteins. Amino acid sequence homology between the SBMV and poliovirus, foot and mouth disease virus, and cowpea mosaic virus reveal that SBMV has a similar genomic organization to picornaviruses with a conserved order: Vpg (Viral protein, genome-linked )-protease-replicase. Two Gln-Ser (QS) amino acid pairs within the 105 KDa polyprotein, C75= 930-936, C60= 1305-1310, are proposed to be the cleavage sites based on 1) similar structural …
Antibody Response To Trypanosoma Cruzi During The Course Of Infection In Mice Held At Elevated Environmental Temperature, Lianyong Gao
Antibody Response To Trypanosoma Cruzi During The Course Of Infection In Mice Held At Elevated Environmental Temperature, Lianyong Gao
Masters Theses & Specialist Projects
It has been demonstrated that changes in environmental temperature can influence the resistance of mice to Trypanosoma cruzi (the causative agent of Chagas' disease in humans) infection. This increased resistance is not only reflected by decreased parasitemias and increased longevity but also by enhanced parasite-specific and nonspecific immune responsiveness in infected mice. The antibody response to heterologous antigen has been demonstrated to be greatly enhanced in infected mice held at elevated temperature. However, the parasite-specific antibody response was reported to be lower in infected mice maintained at 36°C than at room temperature (RT) during early stage of infection, although it …
The Effect Of Dietary Supplemented Vitamin E And Selenium On Experimental Chagas' Disease, Brenda Bennett
The Effect Of Dietary Supplemented Vitamin E And Selenium On Experimental Chagas' Disease, Brenda Bennett
Masters Theses & Specialist Projects
Dietary supplementation with increased levels of vitamin E can enhance the immune system of C3HeB/FeJ mice to enable many of them to survive an otherwise lethal dose of Trypanosoma cruzi, the causative agent of Chagas' disease. To study the role of diet on the course of experimental Chagas' disease, C3HeB/FeJ mice were fed pelleted, synthetic food containing no vitamin E and selenium, 800 IU/kg vitamin E, 2 ppm selenium, or food containing 800 IU/kg vitamin E plus 2 ppm selenium. Mice on synthetic food were compared to control mice fed a commonly used Purina rodent chow # 5001. After diet …
Site-Directed Mutagenesis Of Southern Bean Mosaic Virus (Cowpea Strain): In Search Of A Viral Protease, Alan Simmons
Site-Directed Mutagenesis Of Southern Bean Mosaic Virus (Cowpea Strain): In Search Of A Viral Protease, Alan Simmons
Masters Theses & Specialist Projects
The second and largest open reading frame within the southern bean mosaic virus (SBMV) genome encodes a 105 kDa polyprotein. Following translation, the polyprotein is cleaved to liberate various proteins necessary for SBMV replication. The elements within polyproteins of the picornavirus superfamily members have a conserved order: Vpg (viral protein, genome-linked)-protease-replicase. Amino acid sequence homologies indicate that the 105 kDa protein of SBMV contains a replicase very similar to those identified in polyproteins of the picorna-like viruses. The presence of a VPg covalently attached to the 5' end of the SBMV genome further suggests that SBMV may be considered a …