Medicine and Health Sciences Commons^™

Human Hepatocyte 4-Acetoxy- N, N-Diisopropyltryptamine Metabolite Profiling By Reversed-Phase Liquid Chromatography Coupled With High-Resolution Tandem Mass Spectrometry, Sara Malaca, Marilyn A Huestis, Leonardo Lattanzio, Luigi T Marsella, Adriano Tagliabracci, Jeremy Carlier, Francesco P Busardò

College of Health Professions Faculty Papers

Tryptamine intoxications and fatalities are increasing, although these novel psychoactive substances (NPS) are not controlled in most countries. There are few data on the metabolic pathways and enzymes involved in tryptamine biotransformation. 4-acetoxy-N,N-diisopropyltryptamine (4-AcO-DiPT) is a synthetic tryptamine related to 4-hydroxy-N,N-diisopropyltryptamine (4-OH-DiPT), 4-acetyloxy-N,N-dipropyltryptamine (4-AcO-DPT), and 4-acetoxy-N,N-dimethyltryptamine (4-AcO-DMT). The aim of this study was to determine the best 4-AcO-DiPT metabolites to identify 4-AcO-DiPT consumption through human hepatocyte metabolism and high-resolution mass spectrometry. 4-AcO-DiPT metabolites were predicted in silico with GLORYx freeware to assist in metabolite identification. 4-AcO-DiPT was incubated with 10-donor-pooled human hepatocytes and sample analysis was performed with reversed-phase liquid …