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The Inhibitory Effect Of Bacteriocin Produced By Lactobacillus Acidophilusatcc 4356 And Lactobacillus Plantarum Atcc 8014 On Planktonic Cells And Biofilms Of Serratia Marcescens, Roya Vahedi Shahandashti, Rouha Kasra Kermanshahi, Parinaz Ghadam
The Inhibitory Effect Of Bacteriocin Produced By Lactobacillus Acidophilusatcc 4356 And Lactobacillus Plantarum Atcc 8014 On Planktonic Cells And Biofilms Of Serratia Marcescens, Roya Vahedi Shahandashti, Rouha Kasra Kermanshahi, Parinaz Ghadam
Turkish Journal of Medical Sciences
Background/aim: The spread of antibiotic-resistant pathogens has resulted in the need for new treatments. The aim of the present study is to investigate the effect of bacteriocin from Lactobacillus acidophilus ATCC 4356 and Lactobacillus plantarum ATCC 8014 on planktonic and biofilm forms of Serratia marcescens strains. Materials and methods: The direct antagonism of the L. plantarum and L. acidophilus cell-free supernatant on S. marcescens cultures was determined using an optical density assay. The bacteriocin was partial purified by ammonium sulfate precipitation. Its molecular weight was analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The effect of bacteriocins on the biofilm …
Virulence Factors, Biofilm-Forming Ability, And Antimicrobial Resistance Of Urinary Escherichia Coli Strains Isolated From Hospitalized Patients, Barbara Kot, Jolanta Wicha, Agata Gruzewska, Malgorzata Piechota, Katarzyna Wolska, Maria Obrebska
Virulence Factors, Biofilm-Forming Ability, And Antimicrobial Resistance Of Urinary Escherichia Coli Strains Isolated From Hospitalized Patients, Barbara Kot, Jolanta Wicha, Agata Gruzewska, Malgorzata Piechota, Katarzyna Wolska, Maria Obrebska
Turkish Journal of Medical Sciences
Background/aim: Escherichia coli is the most frequent cause of urinary tract infections. We investigated the possible associations between the origin of strains, antimicrobial resistance, the presence of urovirulence factors, and biofilm-forming ability. Materials and methods: Antibiotic susceptibility of E. coli strains was tested by disk diffusion method. Hemagglutination assays were performed for phenotypic characterization of the cell surface. Multiplex PCR was used for detection of virulence genes and for determination of phylogenetic relationships. Results: The resistance to ampicillin (55.5%) and tetracycline (39.3%) was significantly more frequent than to other antimicrobial agents. The fim gene was present in 92.5% of strains. …