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Full-Text Articles in Medicine and Health Sciences
Evaluation Of New Multiplex Pcr Primers For The Identification Ofplasmodium Species Found In Sabah, Malaysia, Cheronie Shely Stanis, Beng Kah Song, Tock Hing Chua, Yee Ling Lau, Jenarun Jelip
Evaluation Of New Multiplex Pcr Primers For The Identification Ofplasmodium Species Found In Sabah, Malaysia, Cheronie Shely Stanis, Beng Kah Song, Tock Hing Chua, Yee Ling Lau, Jenarun Jelip
Turkish Journal of Medical Sciences
Background/aim: Malaria is a major public health problem, especially in the Southeast Asia region, caused by 5 species of Plasmodium (P. falciparum, P. vivax, P. malariae, P. ovale, and P. knowlesi). The aim of this study was to compare parasite species identification methods using the new multiplex polymerase chain reaction (PCR) against nested PCR and microscopy. Materials and methods: Blood samples on filter papers were subject to conventional PCR methods using primers designed by us in multiplex PCR and previously designed primers of nested PCR. Both sets of results were compared with microscopic identification. Results: Of the 129 samples identified …
An Investigation On The Prevalence And Efficiency Of Immunochromatographic Testing In Suspected Malarial Patients Of Rawalpindi And Islamabad, Pakistan, Saima Jabeen, Sobia Kanwal, Mazhar Qayyum, Umair Farrukh, Shahzad Abdul Hameed
An Investigation On The Prevalence And Efficiency Of Immunochromatographic Testing In Suspected Malarial Patients Of Rawalpindi And Islamabad, Pakistan, Saima Jabeen, Sobia Kanwal, Mazhar Qayyum, Umair Farrukh, Shahzad Abdul Hameed
Turkish Journal of Medical Sciences
Background/aim: The prevalences of Plasmodium falciparum and Plasmodium vivax are increasing rapidly in Pakistan, but recent data on the epidemiology of malaria are not properly reported with scarce diagnostic methods for quick diagnosis. This study was designed to determine the current prevalence and distribution of Plasmodium species in the vicinity of Rawalpindi and Islamabad and report on the validity of the immunochromatographic test (ICT) in diagnosing malarial infections. Materials and methods: A total of 1500 blood samples obtained from a local hospital were screened during the course of this study via microscopic examination and ICT. Results: It was seen that …