Medicine and Health Sciences Commons^™

Phospholipid Scramblase-1-Induced Lipid Reorganization Regulates Compensatory Endocytosis In Neuroendocrine Cells, Stéphane Ory, Mara Ceridono, Fanny Momboisse, Sébastien Houy, Sylvette Chasserot-Golaz, Dimitri Heintz, Valérie Calco, Anna-Marie Haeberlé, Flor A. Espinoza, Peter J. Sims, Yannick Bailly, Marie-France Bader, Stéphane Gasman

Faculty and Research Publications

Calcium-regulated exocytosis in neuroendocrine cells and neurons is accompanied by the redistribution of phosphatidylserine (PS) to the extracellular space, leading to a disruption of plasma membrane asymmetry. How and why outward translocation of PS occurs during secretion are currently unknown. Immunogold labeling on plasma membrane sheets coupled with hierarchical clustering analysis demonstrate that PS translocation occurs at the vicinity of the secretory granule fusion sites. We found that altering the function of the phospholipid scramblase-1 (PLSCR-1) by expressing a PLSCR-1 calcium-insensitive mutant or by using chromaffin cells from PLSCR-1^−/−mice prevents outward translocation of PS in cells stimulated for …