Medicine and Health Sciences Commons^™

Role Of Novel Immunoregulatory Long Noncoding Rnas In Airway Epithelial Pathophysiology And Chronic Pulmonary Disease, Marko Manevski

FIU Electronic Theses and Dissertations

COPD is currently the third leading cause of death globally, accounting for approximately 6% of all deaths in 2019, and cigarette smoke (CS) is the primary risk factor for disease development.

Transcriptomic analysis of a 3D in vitro model using differentiated human airway epithelial cells (AECs) identified a novel lncRNA on the antisense strand of ICAM-1 or LASI that showed increased expression upon CS exposure. The lncRNA was significantly upregulated in CS-induced Rhesus macaque airways and in human COPD airways that exhibited higher mucus expression and goblet cell hyperplasia, which was recapitulated in vitro. Blocking lncRNA expression in cell culture …

Specific Increase In Mdr1 Mediated Drug-Efflux In Human Brain Endothelial Cells Following Co-Exposure To Hiv-1 And Saquinavir, Upal Roy, Christine Bulot, Kerstin Honer Zu Bentrup, Debasis Mondal

HWCOM Faculty Publications

Persistence of HIV-1 reservoirs within the Central Nervous System (CNS) remains a significant challenge to the efficacy of potent anti-HIV-1 drugs. The primary human Brain Microvascular Endothelial Cells (HBMVEC) constitutes the Blood Brain Barrier (BBB) which interferes with anti-HIV drug delivery into the CNS. The ATP binding cassette (ABC) transporters expressed on HBMVEC can efflux HIV-1 protease inhibitors (HPI), enabling the persistence of HIV-1 in CNS. Constitutive low level expression of several ABC-transporters, such as MDR1 (a.k.a. P-gp) and MRPs are documented in HBMVEC. Although it is recognized that inflammatory cytokines and exposure to xenobiotic drug substrates (e.g HPI) can …