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Medicine and Health Sciences Commons

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Medical Sciences

Florida International University

Series

2002

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Full-Text Articles in Medicine and Health Sciences

D1s80 Dna Profiling In Five African Populations, Leslie R. Adrien Jul 2002

D1s80 Dna Profiling In Five African Populations, Leslie R. Adrien

FIU Electronic Theses and Dissertations

The highly polymorphic DlS80 locus has no known genetic function. This variable number of tandem repeat (VNTR) has been valuable in forensic identification. We have obtained allelic and genotypic frequencies for five African populations (Benin, Cameroon, Egypt, Kenya and Rwanda), which could be employed as databases to identify individuals.

The polymerase chain reaction, followed by vertical polyacrylamide gel electrophoresis and silver staining was our method of analysis. Allele frequencies were used to infer genetic associations using Phylip 3.5, Principal Component and G-test statistical programs. Tests for Hardy-Weinberg equilibrium were employed. Fst estimates and power of discrimination values were also determined …