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Studies On Antibody Production. Viii. The Inhibitory Effect Of Chloramphenicol On The Synthesis Of Antibody In Tissue Culture, Charles T. Ambrose, Albert H. Coons
Studies On Antibody Production. Viii. The Inhibitory Effect Of Chloramphenicol On The Synthesis Of Antibody In Tissue Culture, Charles T. Ambrose, Albert H. Coons
Charles T. Ambrose
In 1953 Gale and Folkes (1) observed that chloramphenicol inhibited protein synthesis in Staphylococcus aureus but permitted substantial nucleic acid synthesis to continue. Subsequently, this drug has served as a powerful tool in studies of protein and nucleic acid metabolism in a wide variety of microbial systems. In sensitive bacterial cultures the addition of 30 to 100/~g/ml 0.1-0.31m~) of chloramphenicol results in prompt and nearly complete suppression of protein synthesis (1, 2), while concentrations as low as 2 ~g/ml (0.006 m~) produce 50 per cent inhibition in some bacteria (1). Very low oncentrations also inhibit protein synthesis in cell-free bacterial …
Antibody Production In Organ Cultures Of Lymph-Node Fragments Following In Vitro Secondary Antigenic Stimulation, Charles T. Ambrose
Antibody Production In Organ Cultures Of Lymph-Node Fragments Following In Vitro Secondary Antigenic Stimulation, Charles T. Ambrose
Charles T. Ambrose
The technique for obtaining a secondary antibody response in vitro by stimulating cultured fragments of immune rabbit lymph nodes is described. A thin pad of glass wool overlying the fragments in a Leighton tube was used to hold the fragments in place and to replace plasma for this function. In addition, the glass wool fibers provided an increased surface area for cellular migration, which may be studied in living cultures or in fixed and stained preparations. Experiments leading to the development of chemically defined medium are described; the components of this medium that appear to substitute for serum include hydrocortisone …