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Full-Text Articles in Medicine and Health Sciences
The Mauc Gene Encodes A Versatile Signal Sequence And Redox Protein That Can Be Utilized In Native And Non-Native Protein Expression And Electron Trnasfer Systems, Brian Dow
Electronic Theses and Dissertations
The redox-active type 1 copper site of amicyanin is composed of a single copper ion that is coordinated by two histidines, a methionine, and a cysteine residue. This redox site has a potential of +265 mV at pH7.5. Over ten angstroms away from the copper site resides a tryptophan residue whose fluorescence is quenched by the copper. The effects of the tryptophan on the electron transfer (ET) properties were investigated by site-directed mutagenesis. Lessons learned about the hydrogen bonding network of amicyanin from the aforementioned study were attempted to be used as a model to increase the stability of another …
Structural And Functional Characterization Of Enzymes Of A Novel Group Of Tryptophylquinone Cofactor Containing Oxidases, Esha Sehanobish
Structural And Functional Characterization Of Enzymes Of A Novel Group Of Tryptophylquinone Cofactor Containing Oxidases, Esha Sehanobish
Electronic Theses and Dissertations
Protein-derived cofactors are redox and catalytic centers that are generally formed by the posttranslational modifications of one or more amino acids. An important class of these cofactors are the quinones derived from tyrosine and tryptophan. Amongst redox proteins, it has been known till now that oxidases either contain a flavin or a tyrosylquinone cofactor, whereas tryptophylquinone ones are present within the dehydrogenases. In recent times, oxidases from a marine bacterium, Marinomonas mediterranea, have been shown to possess the latter. This study involves the characterization of two such proteins, lysine-?-oxidase (LodA) and glycine oxidase (GoxA). They have been reported to contain …
Role Of Klf8-Cxcr4 Signaling In Breast Cancer Metastasis, Debarati Mukherjee
Role Of Klf8-Cxcr4 Signaling In Breast Cancer Metastasis, Debarati Mukherjee
Electronic Theses and Dissertations
Krüppel-like factor 8 (KLF8) has been strongly implicated in breast cancer metastasis. However, the underlying mechanisms remain largely unknown. In this study we report a novel signaling from KLF8 to C-X-C cytokine receptor type 4 (CXCR4) in breast cancer. Overexpression of KLF8 in MCF-10A cells induced CXCR4 expression at both mRNA and protein levels. This induction was well correlated with increased Boyden chamber migration, matrigel invasion and transendothelial migration (TEM) of the cells towards the ligand CXCL12. On the other hand, knockdown of KLF8 in MDA-MB-231 cells reduced CXCR4 expression associated with decreased cell migration, invasion and TEM towards CXCL12. …
Biophysical Sources Of 1/F Noises In Neurological Tissue, Alan Paris
Biophysical Sources Of 1/F Noises In Neurological Tissue, Alan Paris
Electronic Theses and Dissertations
High levels of random noise are a defining characteristic of neurological signals at all levels, from individual neurons up to electroencephalograms (EEG). These random signals degrade the performance of many methods of neuroengineering and medical neuroscience. Understanding this noise also is essential for applications such as real-time brain-computer interfaces (BCIs), which must make accurate control decisions from very short data epochs. The major type of neurological noise is of the so-called 1/f-type, whose origins and statistical nature has remained unexplained for decades. This research provides the first simple explanation of 1/f-type neurological noise based on biophysical fundamentals. In addition, noise …
The Role Of Sod1 Acetylation In Neurodegeneration, Michael Kaliszewski
The Role Of Sod1 Acetylation In Neurodegeneration, Michael Kaliszewski
Electronic Theses and Dissertations
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder affecting motor neurons. Cu, Zn superoxide dismutase (SOD1), a cytoplasmic free radical scavenging enzyme, is mutated in familial ALS (fALS) and post-translational modification of the wild-type protein has been associated with sporadic ALS (sALS). Proteomic studies indicate that SOD1 is acetylated at Lys123; however, the role of this modification remains unknown. To investigate its function, we generated antibodies for Lys123-acetylated SOD1 (Ac-K123 SOD1). Sod1 deletion in Sod1-/- mice, K123 mutation, or preabsorption with Ac-K123 peptide suppressed immunoreactivity, confirming antibody specificity. In the normal central nervous system, Ac-K123 SOD1 maps to glutamatergic …