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Detection Of Pathogenic Leptospira Spp. By Rpa-Nalfia Targeting Lipl32 Gene, Nattachai Srisawat
Detection Of Pathogenic Leptospira Spp. By Rpa-Nalfia Targeting Lipl32 Gene, Nattachai Srisawat
Chulalongkorn Medical Journal
Background: Lack of available sensitive point-of-care tests is one of the key challenges limiting the early point-of-care diagnosis of leptospirosis.Previously, a Recombinase Polymerase Amplification (RPA) and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 12a (CRISPR/Cas12a) lipL32 detection platform with high sensitivity and specificity was developed. However, its turnaround time is between one and two hours, and two reactions are required.
Objective: To develop the RPA in combination with a nucleic acid lateral flow immunoassay (NALFIA) detection platform to reduce the turnaround time and make it a one-step reaction.
Methods: RPA combined with nucleic acid lateral flow immunoassay (NALFIA) detection …