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Distinct Progenitor Origin Distinguishes A Lineage Of Dendritic-Like Cells In Spleen., Sawang Petvises, Helen O'Neill
Distinct Progenitor Origin Distinguishes A Lineage Of Dendritic-Like Cells In Spleen., Sawang Petvises, Helen O'Neill
Helen O'Neill
The dendritic cell (DC) compartment comprises subsets of cells with distinct phenotypes. Previously this lab reported methodology for hematopoiesis of dendritic-like cells in vitro dependent on a murine splenic stromal cell line (5G3). Co-cultures of lineage-depleted bone marrow (Lin- BM) over 5G3 continuously produced a distinct population of dendritic-like "L-DC" for up to 35 days. Here the progenitor of L-DC is investigated in relation to known BM-derived hematopoietic progenitors. It is shown here that L-DC-like cells also derive from the CD150+Flt3- long-term reconstituting-hematopoietic stem cells (HSC), and also from the Flt3+ multipotential progenitor subset in BM. Lin- BM co-cultures also …