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Full-Text Articles in Medicine and Health Sciences
A Transgenic Pig Model Expressing A Cmv-Zsgreen1 Reporter Across An Extensive Array Of Tissues., Amy T Desaulniers, Rebecca A Cederberg, Elizabeth P Carreiro, Channabasavaiah B Gurumurthy, Brett R White
A Transgenic Pig Model Expressing A Cmv-Zsgreen1 Reporter Across An Extensive Array Of Tissues., Amy T Desaulniers, Rebecca A Cederberg, Elizabeth P Carreiro, Channabasavaiah B Gurumurthy, Brett R White
Journal Articles: Pharmacology & Experimental Neuroscience
Since genetic engineering of pigs can benefit both biomedicine and agriculture, selecting a suitable gene promoter is critically important. The cytomegalovirus (CMV) promoter, which can robustly drive ubiquitous transgene expression, is commonly used at present, yet recent reports suggest tissue-specific activity in the pig. The objective of this study was to quantify ZsGreen1 protein (in lieu of CMV promoter activity) in tissues from pigs harboring a CMV-ZsGreen1 transgene with a single integration site. Tissue samples (
Engineered Extracellular Vesicles Loaded With Mir-124 Attenuate Cocaine-Mediated Activation Of Microglia, Ernest T. Chivero, Ke Liao, Fang Niu, Ashutosh Tripathi, Changhai Tian, Shilpa Buch, Guoku Hu
Engineered Extracellular Vesicles Loaded With Mir-124 Attenuate Cocaine-Mediated Activation Of Microglia, Ernest T. Chivero, Ke Liao, Fang Niu, Ashutosh Tripathi, Changhai Tian, Shilpa Buch, Guoku Hu
Journal Articles: Pharmacology & Experimental Neuroscience
MicroRNA-124 (miR-124), a brain-enriched microRNA, is known to regulate microglial quiescence. Psychostimulants such as cocaine have been shown to activate microglia by downregulating miR-124, leading, in turn, to neuroinflammation. We thus rationalized that restoring the levels of miR-124 could function as a potential therapeutic approach for cocaine-mediated neuroinflammation. Delivering miRNA based drugs in the brain that are effective and less invasive, however, remains a major challenge in the field. Herein we engineered extracellular vesicles (EVs) and loaded them with miR-124 for delivery in the brain. Approach involved co-transfection of mouse dendritic cells with Dicer siRNA and RVG-Lamp2b plasmid to deplete …