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Full-Text Articles in Medicine and Health Sciences
The Cytoplasmic Tail Of The Rabies Virus G Protein Is An Essential Domain Controlling Death/Survival In Human Neuronal Cells, Christophe Prehaud, Mireille Lafage, Gene S. Tan, Françoise Mégret, Pauline Ménager, Matthias Schnell, Henri Buc, Monique Lafon
The Cytoplasmic Tail Of The Rabies Virus G Protein Is An Essential Domain Controlling Death/Survival In Human Neuronal Cells, Christophe Prehaud, Mireille Lafage, Gene S. Tan, Françoise Mégret, Pauline Ménager, Matthias Schnell, Henri Buc, Monique Lafon
Department of Microbiology and Immunology Faculty Papers
Poster presentation.
Signal Transducer And Activator Of Transcription (Stat)5 Activation By Bcr/Abl Is Dependent On Intact Src Homology (Sh)3 And Sh2 Domains Of Bcr/Abl And Is Required For Leukemogenesis., M Nieborowska-Skorska, M A Wasik, A Slupianek, P Salomoni, T Kitamura, B Calabretta, T Skorski
Signal Transducer And Activator Of Transcription (Stat)5 Activation By Bcr/Abl Is Dependent On Intact Src Homology (Sh)3 And Sh2 Domains Of Bcr/Abl And Is Required For Leukemogenesis., M Nieborowska-Skorska, M A Wasik, A Slupianek, P Salomoni, T Kitamura, B Calabretta, T Skorski
Department of Microbiology and Immunology Faculty Papers
Signal transducer and activator of transcription (STAT)5 is constitutively activated in BCR/ ABL-expressing cells, but the mechanisms and functional consequences of such activation are unknown. We show here that BCR/ABL induces phosphorylation and activation of STAT5 by a mechanism that requires the BCR/ABL Src homology (SH)2 domain and the proline-rich binding site of the SH3 domain. Upon expression in 32Dcl3 growth factor-dependent myeloid precursor cells, STAT5 activation-deficient BCR/ABL SH3+SH2 domain mutants functioned as tyrosine kinase and activated Ras, but failed to protect from apoptosis induced by withdrawal of interleukin 3 and/or serum and did not induce leukemia in severe combined …
Expression Of Constitutively Active Raf-1 In The Mitochondria Restores Antiapoptotic And Leukemogenic Potential Of A Transformation-Deficient Bcr/Abl Mutant., P Salomoni, M A Wasik, R F Riedel, K Reiss, J K Choi, T Skorski, B Calabretta
Expression Of Constitutively Active Raf-1 In The Mitochondria Restores Antiapoptotic And Leukemogenic Potential Of A Transformation-Deficient Bcr/Abl Mutant., P Salomoni, M A Wasik, R F Riedel, K Reiss, J K Choi, T Skorski, B Calabretta
Department of Microbiology and Immunology Faculty Papers
The oncogenic BCR/ABL protein protects hematopoietic cells from apoptosis induced by growth factor deprivation, but the mechanisms are only partially understood. A BCR/ABL mutant lacking amino acids 176-426 in the BCR domain (p185DeltaBCR) failed to protect interleukin 3-deprived 32Dcl3 myeloid precursor cells from apoptosis, although it possessed tyrosine kinase activity and was capable of activating the Ras-Raf-MAP kinase pathway. Compared to p185 wild-type transfectants, p185DeltaBCR-transfected cells showed markedly reduced levels of Bcl-2 and expressed the hypophosphorylated, proapoptotic form of BAD. Bcl-2 expression in the mitochondrial fraction of p185DeltaBCR cells was also markedly diminished and mitochondrial RAF was undetectable. In p185DeltaBCR …
A Tandem Duplication Within The Fibrillin 1 Gene Is Associated With The Mouse Tight Skin Mutation., Linda D. Siracusa, Rodney Mcgrath, Qing Ma, John J. Moskow, Jayanthi Manne, Paul J. Christner, Arthur M. Buchberg, Sergio A. Jimenez
A Tandem Duplication Within The Fibrillin 1 Gene Is Associated With The Mouse Tight Skin Mutation., Linda D. Siracusa, Rodney Mcgrath, Qing Ma, John J. Moskow, Jayanthi Manne, Paul J. Christner, Arthur M. Buchberg, Sergio A. Jimenez
Department of Medicine Faculty Papers
Mice carrying the Tight skin (Tsk) mutation have thickened skin and visceral fibrosis resulting from an accumulation of extracellular matrix molecules. These and other connective tissue abnormalities have made Tskl + mice models for scleroderma, hereditary emphysema, and myocardial hypertrophy. Previously we localized Tsk to mouse chromosome 2 in a region syntenic with human chromosome 15. The microfibrillar glycoprotein gene, fibrillin 1 (FBN1), on human chromosome 15q, provided a candidate for the Tsk mutation. We now demonstrate that the Tsk chromosome harbors a 30- to 40-kb genomic duplication within the Fbn1 gene that results in a larger than normal in-frame …