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Full-Text Articles in Medicine and Health Sciences
Inhibition Of Enterococcus Faecium Adherence To Collagen By Antibodies Against High-Affinity Binding Subdomains Of Acm, Sreedhar R Nallapareddy, Jouko Sillanpää, Vannakambadi K Ganesh, Magnus Höök, Barbara E Murray
Inhibition Of Enterococcus Faecium Adherence To Collagen By Antibodies Against High-Affinity Binding Subdomains Of Acm, Sreedhar R Nallapareddy, Jouko Sillanpää, Vannakambadi K Ganesh, Magnus Höök, Barbara E Murray
Journal Articles
Strains of Enterococcus faecium express a cell wall-anchored protein, Acm, which mediates adherence to collagen. Here, we (i) identify the minimal and high-affinity binding subsegments of Acm and (ii) show that anti-Acm immunoglobulin Gs (IgGs) purified against these subsegments reduced E. faecium TX2535 strain collagen adherence up to 73 and 50%, respectively, significantly more than the total IgGs against the full-length Acm A domain (28%) (P < 0.0001). Blocking Acm adherence with functional subsegment-specific antibodies raises the possibility of their use as therapeutic or prophylactic agents.